Chemical Proteomics provides key techniques to investigate chemical proteomics. The text presents analytical strategies, how probe...
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Chemical Proteomics provides key techniques to investigate chemical proteomics. The text presents analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery.
The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function. In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chemical Proteomics : Methods and Protocols seeks to provide methodologies that will contribute to a wider application of chemical proteomics methods in biochemical and cell biological laboratories.
Contributes to a wider application of chemical proteomics methods in biochemical and cell biological laboratories Provides step-by-step detail essential for reprodicible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras Inhalt Part I: Introduction 1. Mass Spectrometry-Based Chemoproteomic ApproachesMarcus Bantscheff2. Chemical Proteomics in Drug DiscoveryGerard DrewesPart II: Small Molecules and Probe Design3. Compound Immobilization and Drug-Affinity ChromatographyUwe Rix, Manuela Gridling, and Giulio Superti-Furga4. Affinity-based Chemoproteomics with Small Molecule-peptide ConjugatesChaitanya Saxena5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol RhodanineXia Ge and Daniel S. Sem6. Probing Proteomes with Benzophenone PhotoprobesAkira Kawamura and Doina M. Mihai7. Biotinylated Probes for the Analysis of Protein Modification by ElectrophilesSimona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler8. Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS) Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias DregerPart III: Target Discovery and Target Validation 9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass TagsCarsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff11. Affinity-Based Profiling of Dehydrogenase SubproteomesXia Ge and Daniel S. Sem12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten13. Fluorescence-Based Proteasome Activity Profiling Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers14. Chemical Cross-Linking and High Resolution Mass Spectrometry to Study Protein-Drug Interactions Mathias Q. Müller and Andrea Sinz15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI Mass SpectrometryNatalia Gasilova, Alexis Nazabal16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome StudiesAmy Hye Won Jeon, Gerold Schmitt-UlmsPart IV: Ligand Discovery17. Ligand Discovery Using Small-molecule MicroarraysDominick E. Casalena, Dina Wassaf, and Angela N. Koehler18. Working with Small-Molecules-Preparing and Storing Stock Solutions and Determination of Kinetic Solubility Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard19. A Database for Chemical Proteomics-ChEBIPaula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck20. Working with Small Molecules: Rules-of-Thumb of Drug LikenessMing-Qiang Zhang