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Cell-Penetrating Peptides

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Recently, a new understanding of cell-penetrating peptides has emerged. In this book, expert researchers explore the latest inform... Weiterlesen
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Recently, a new understanding of cell-penetrating peptides has emerged. In this book, expert researchers explore the latest information on cell-penetrating peptides (CPPs). They provide insight into the most important and contemporary areas of CPP research.

In recent years, a new understanding of cell-penetrating peptides has emerged, helping researchers to expand beyond a number of long-held dogmas. In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating peptides (CPPs), providing insight into the most important and contemporary areas of CPP research. Chapters address the historical background of CPP studies, provide an overview of the growing field of research, investigate methods for testing CPP mechanisms, present a summary of methods that attempt to use properties of CPPs to study biochemical intracellular mechanisms of interaction and signal transduction, and include new ideas for turning CPP-based strategies into drugs. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and current, Cell-Penetrating Peptides: Methods and Protocols is an essential guide that allows researchers to study intracellular mechanisms in new ways, and to promote the future development of novel drugs.

From the reviews:

Prof. Ulo Langel rightly explain in a necessary preface that there are in use so many definition for the same things: protein transduction domain (PTDs), Trojan peptides, model amphipathic peptides (MAPs), membrane translocating sequences (MTS) that the best way to refer to all of these molecules is to call all of them cell-penetrating peptides, CPPs. Clearly enough, the cell-penetrating peptides volume is surely of great help for basic research and for clinicians engaged in translational medicine. (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)


Cell-penetrating Peptides. Methods and Protocols.
Ulo Langel, Editor
Table of Contents:

Table of Contents



Part I. Introduction
1. Classes and prediction of cell-penetrating peptides
Maria Lindgren and Ülo Langel

2. Penetratin story : an overview
Edmond Dupont, Alain Prochiantz and Alain Joliot

Part II. Methods to test mechanisms of cell-penetrating peptides
3. Testing membrane interactions of CPPs
Astrid Gräslund and Lena Mäler

4. Interactions of amphipathic CPPs with model membranes
Sébastien Deshayes, Karidia Konate, Gudrun Aldrian, Frédéric Heitz, and Gilles Divita

5. NMR studies of three-dimensional structure and positioning of CPPs in membrane model systems
Lena Mäler and Astrid Gräslund

6. Measurements of the intracellular stability of CPPs
Ivo R. Ruttekolk, Wouter P. R. Verdurmen, Yi-Da Chung, and Roland Brock

7. Tools for predicting binding and insertion of CPPs into lipid bilayers
Paulo Almeida

8. Studies of proteoglycan involvement in CPP-mediated delivery
Anders Wittrup, Si-He Zhang and Mattias Belting

9. Uptake Kinetics of cell-penetrating peptides
Anders Florén, Imre Mäger, and Ülo Langel

10. Thermodynamics of lipid interactions with cell-penetrating peptides
Reto Sauder, Joachim Seelig and André Ziegler

11. Calcium and membrane repair
Caroline Palm-Apergi, and Mattias Hällbrink

12. Mapping of protein transduction pathways with fluorescent microscopy
Helin Räägel, Pille Säälik, Ülo Langel, and Margus Pooga

13. Insight into cell-entry mechanisms of CPPs by electron microscopy
Kärt Padari, Annely Lorents, Eija Jokitalo and Margus Pooga

14. Toxicity methods for CPPs
Per Lundin, Samir EL Andaloussi and Ülo Langel

15. Comparison of CPP uptake methods
Tina Holm, Samir EL Andaloussi, and Ülo Langel

16. Characterization of cellular internalization pathways for CPP-mediated oligonucleotide delivery
Peter Guterstam, Samir EL Andaloussi and Ülo Langel

Part III. Functionality and cell-penetrating peptides
17. Mimicry of protein function with cell-penetrating peptides
Henrik Johansson, Samir EL Andaloussi and Ülo Langel

18. Homeoprotein intercellular transfer, the hidden face of cell-penetrating peptides
Alain Prochiantz

19. Pharmacology, biodistribution, and efficacy of GPCR-based pepducins in disease models
Sarah L. Tressel, Georgios Koukos, Boris Tchernychev, Suzanne L. Jacques, Lidija Covic, Athan Kuliopulos

20. Identification and Characterization of Tissue-Specific Protein Transduction Domains using Peptide Phage Display
Maliha Zahid, and Paul D. Robbins

21. Applications of Cell Penetrating Peptides as Signal Transduction Modulators for the Selective Induction of Apoptosis
Sarah Jones and John Howl

Part IV. Applications of CPPs in gene modulation
22. Splice Redirection as a Convenient Assay to Monitor CPP-ON Efficiency and Mechanism
Rachida Abes, Andrey A. Arzumanov, Amer F. Saleh, Fatouma Said Hassane, Michael J. Gait and Bernard Lebleu

23. CPP directed oligonucleotide exon skipping in animal models of Duchenne muscular
HaiFang Yin, Hong Moulton, Corinne Betts and Matthew Wood

24. PTD-DRBD siRNA delivery
Caroline Palm-Apergi, Akiko Eguchi, and Steven F. Dowdy

25. A non-covalent peptide-based strategy for siRNA delivery
Laurence Crombez, and Gilles Divita

26. Application of PepFect peptides for the delivery of splice correcting oligonucleotides
Samir EL Andaloussi, Taavi Lehto, Per Lundin, and Ülo Langel

27. Internalization of nucleoside phosphates into live cells by complex formation with
different CPPs and JBS-Nucleoducin
Franziska Mussbach, Regina Pietrucha, Buerk Schaefer and Siegmund Reissmann

28. Enhanced cellular delivery of cell-penetrating peptide -peptide nucleic acid conjugates by photochemical internalization (PCI).
Takehiko Shiraishi, and Peter E. Nielsen

Part V. CPP in vivo and as future drugs
29. Identification of homing peptides using the in vivo phage display technology
Antti Rivinoja and Pirjo Laakkonen

30. Measuring the action of CPP-siRNA conjugates in the lung
Sterghios A. Moschos, Karen G. Spink, and Mark A. Lindsay

31. Intracellular delivery of nanoparticles with CPPs
Rupa Sawant, Vladimir Torchilin

32. Multifunctional CPP polymer system for tumor-targeted pDNA and siRNA delivery
Christian Dohmen, Ernst Wagner

33. Cell Penetrating Penta-Peptides (CPP5s) and Bax Inhibiting Peptides (BIPs): Protocol for their application
Jose Gomez and Shigemi Matsuyama

34. PAIR technology: exon-specific RNA binding protein isolation in live cells
Thomas J. Bell, Emelía Eiríksdóttir, Ülo Langel and James Eberwine

35. Quantitation of cellular and topical uptake of luciferin oligoarginine conjugates
Jonathan B. Rothbard and Lisa R. Jones

36. Industrial-scale manufacturing of a possible oligonucleotide cargo CPP based drug
Ulf Tedebark, Anthony Scozzari, Oleg Werbitzky, Daniel Capaldi and Lars Holmberg

37. Application of a fusiogenic peptide GALA for intracellular delivery
Ikuhiko Nakase, Kentaro Kogure, Hideyoshi Harashima, Shiroh Futaki

38. Therapeutic Applications of Cell-Penetrating Peptides
Randolph M. Johnson, Stephen D. Harrison and Derek Maclean

39. Nonclinical and clinical experiences with CPP-based self assembling peptide systems in
topical drug development
Jacob M. Waugh, Jane Lee, Michael D. Dake and Dan Browne


Titel: Cell-Penetrating Peptides
Untertitel: Methods and Protocols
EAN: 9781493961559
ISBN: 1493961551
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 604
Gewicht: 1119g
Größe: H254mm x B178mm x T32mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2011

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