Cell Migration

Cell migration is a key component of many biological processes. This book provides a catalogue of techniques for the study of cell migration. It brings together a wide range of these techniques from the basic migration assays to recent technical advances.

Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study. The aim of Cell Migration: Developmental Methods and Protocols , Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances. Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D. It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Cell Migration: Developmental Methods and Protocols, Second Edition provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for anyresearcher who wishes to explore this significant area of cell biology.

Provides a comprehensive catalogue of techniques for the study of cell migration Includes step-by-step, readily reproducible protocols Features key tips and expert implementation advice for successful results

Inhalt

Cell Migration: An Overview.- Scratch-Wound Assay.- HGF-Induced DU145 Cell Scatter Assay.- Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time.- Imaging Cells Within 3D Cell Derived Matrix.- Live Cell Imaging of Neuronal Growth Cone Motility and Guidance In Vitro.- Boyden Chamber.- Transwell® Invasion Assays.- Imaging Podosome Dynamics and Matrix Degradation.- Endothelial Cell Migration Under Flow.- In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments.- Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy.- Chemotaxis of Slow Migrating Mammalian Cells Analyzed by Video Microscopy.- Live Cell Fluorescence Microscopy Techniques.- Measuring Invasion in an Organotypic Model.- Analysis of Cell Migration Using Caenorhabditis elegans as a Model System.- Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration.- Measuring Inflammatory Cell Migration in the Zebrafish.- Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement.- Assessment of Development and Chemotaxis in Dictyostelium discoideum Mutants.- Simple Experimental and Spontaneous Metastasis Assays in Mice.- Two-Photon Intravital Multicolour Imaging to Study Metastatic Behaviour of Cancer Cells In Vivo.- Measuring Angiogenesis in Mice.- Time-Lapse Imaging of Chick Cardiac Precursor Cells.- Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy.- Fluorescence Recovery After Photobleaching.- Measuring FRET Using Time-Resolved FLIM.- Cell Migration in Confinement: A Micro-Channel Based Assay.-Functional Screening with a Live Cell Imaging Based Random Cell Migration Assay.- Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays.