Cell-Based Microarrays

As a high-throughput method of analyzing gene function, cell-based microarrays have proven of vital importance. Here, experts provide an up-to-date assessment of these microarrays and meticulous coverage of all their aspects, including emerging technology.

As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.

Provides an up-to-date variety of protocols covering all aspects in thorough, clear detail -Presents readers with clear step-by-step protocols to allow them to set up difficult techniques and expand upon them -Includes tips from the experts highlighting potential methodological pitfalls in order to ensure successful implementation Includes supplementary material: sn.pub/extras

Inhalt
Cell-Based Microarrays: Overview.- Cell-Based Co-Transfection Microarrays for Use with HEK293T Cells on a Poly D-Lysine Polystyrene Coated Microplate.- Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays.- A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays.- High-Throughput Subcellular Protein Localization Using Transfected-Cell Arrays.- Cell Arrays for the Measurement of Organelle Dynamics in Living Cells.- High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays.- Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA).- Reverse Transfected Cell Microarrays in Infectious Disease Research.- Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies.- Blood Cell Capture on Antibody Micro-Arrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance Imaging.- Immobilized Culture and Transfection Microarray of Nonadherent Cells.- Plasma Polymer and PEG-Based Coatings for DNA, Protein, and Cell Microarrays.- Polymer Microarrays for Cellular High-Content Screening.- High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation.- Microfluidic Image Cytometry.