Cell and Molecular Biology of Artemia Development

The brine shrimp Artemia has become an important experimental system for studies of the developmental process. In recent years the shrimp has yielded considerable information on the pattern of development, bio chemistry, and gene structure and expression of crustaceans. This book is a compilation of research activity from twenty five of the most active re search laboratories working with brine shrimp in the above areas. It also represents the proceedings of a NATO Advanced Research Workshop held in Montreal, Canada, August 11-13, 1988. The book contains twenty nine full papers covering the major areas discussed at the workshop. In addition, one page abstracts representing seventeen poster presentations which were given at the workshop, and which were deemed to be most relevant to the theme of the book, are included. These are designated with an [al in the Table of Contents following the title of each paper. A considerable amount of discussion which took place during the workshop has not been included in the book because of space limitations. However, the editors will endeavour to make some of this in formation available at a later date through the Artemia Newsletter. In addition to the high percentage of invited speakers who attended and contributed to the workshop, the organizers would like to thank a number of participants who made valuable contributions to the major dis cussion sessions. These include: John Freeman, Michael Horst, Herman Slegers, Jack Vaughn, Frank Conte, Sandy McLennan, Clive Trotman and Patrick Sorgeloos.

Inhalt
Dormancy and Development.- Aspects of the Anaerobic Metabolism of Artemia Cysts.- Developmental Abnormalities Related to Bicarbonate Ion Status during Emergence of Artemia.- Light-Induced Development of Artemia Cysts.- Adaption to Hypoxia in Artemia.- Oxygen Binding in Artemia Hemolymph [a]*.- Disruption of Artemia Development by Metals [a].- Cellular and Structural Aspects.- Molecular and Cellular Aspects of Chitin Synthesis in Larval Artemia.- Segment Morphogenesis in Artemia Larvae.- Heat Shock Induces Developmental Anomalies in Artemia.- Morphological Study of the Ovary of Artemia.- Enzyme Markers in Development: Cholinesterase (ChE), Acid Hydrolases, Alkaline Phosphatase (ALP) and Aminopeptidase (AP) in Embryos and Larvae of Artemia.- Multiple Protease Inhibitors in Artemia Cysts.- Effect of Starvation on Amylase and Trypsin Activities at two Developmental Stages of Artemia (San Francisco) [a].- Artemia Trehalase: Regulation by Factors That also Control Resumption of Development.- Chitin Degrading Enzymes: Characterization and Functions during Artemia Development.- Regulation of Artemia Glycogen Phosphorylase: Effect of Diguanosine Nucleotides [a].- Metalloproteins in Developing Artemia.- A New Method for the Purification of Nuclei from Artemia [a].- Enzymes of Dinucleoside Oligophosphate Metabolism in Artemia Cysts and Larvae.- Comparison of Diguanosine Polyphosphate Levels in Various Populations of Artemia Cysts [a].- Phosphonate Analogues of Diadenosine Tetraphosphate as Substrates and Inhibitors of Artemia Dinucleoside Tetraphosphatase.- Purification and Characterization of a Phthalate Ester Hydrolyzing Enzyme from Artemia [a].- Messenger Ribonucleoproteins of Cryptobiotic Gastrulae of Artemia: Mechanisms of Activation and Repression of Non-Polysomal MessengerRibonucleoproteins.- Kinetic and Thermodynamic Parameters of Artemia Ribosome Subunit Interactions: The Effects of Polyamines.- Study of Poly(ADP-ribosyl)ation in Artemia Embryos at Various Stages of Development [a].- Adventures and Misadventures in the Genome of Artemia.- Structure and Expression of Ribosomal RNA Genes from Artemia.- Ribosomal RNA Genes during Development in the Brine Shrimp, Artemia: Structure, Expression and Evolution.- Species and Population Polymorphism of the Histone and 5S Gene Repeat Unit in Artemia [a].- Organization of 5S-Histone Genes in Different Artemia Populations [a].- cDNA Cloning of Developmentally Regulated Artemia Genes.- Artemia Mitochondrial DNA.- Identification of a Protein Which Specifically Binds a Highly Repetitive Heterochromatic DNA Family (Alu 1 Family) of Artemia franciscana.- Functional and Structural Studies on Artemia Polypeptide Chain Initiation Factor 2: Cloning and Sequencing of eIF-2 ? cDNA.- Structure of Ubiquitin Genes in Artemia [a].- Isolation of cDNA Clones Coding for Artemia Type P ATPases [a].- Structure and Expression during Development of Artemia c-ras Genes [a].- Molecular Biology of Larval Osmoregulation.- Differential Gene Expression of Na, K-ATPase ? and ? -Subunits in the Developing Brine Shrimp, Artemia.- Tubulins and Hemoglobins.- Tubulin Synthesis and Microtubule Organization in Artemia.- Repeated Sequences Flank the Tubulin Genes of Artemia [a].- Microtubule Cross-Linking Proteins from Artemia [a].- The Diversity of ? -Tubulin during Artemia Prelarval Development [a].- The Molecular Biology of Artemia Haemoglobins.- Localization of Putative Haemoglobin by Immunogold Staining in Artemia [a].- The Structure of Artemia Hemoglobin and Hemoglobin Domains.- Computer Searches.- Computer Utilizationin Artemia Research.- Contributors.