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Cardiovascular Proteomics

  • Kartonierter Einband
  • 432 Seiten
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This cutting-edge book presents protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering ... Weiterlesen
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Beschreibung

This cutting-edge book presents protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering researchers in the field. Topics explored in this comprehensive volume include obtaining specific heart proteins, techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels.

Inhalt

Part I. Analysis of Heart Proteome Two-dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomics Michael J. Dunn Analyzing The Cardiac Muscle Proteome By Liquid Chromatography-Mass Spectrometry (Lc-ms) Based Expression Proteomics Anthony O. Gramolini, Thomas Kislinger, Peter Liu, David H. Maclennan, and Andrew Emili 2-D Differential Gel Electrophoresis (DIGE) of Rat Heart Proteins in Ischemia and Ischemia-Reperfusion Jun Sakai, Hironori Ishikawa, Hideshi Satoh, Setsuko Yamamoto, Shinichi Kojima, and Masaharu Kanoaka Analysis of Antihypertensive Drugs in the Heart of Animal Models: A Proteomic Approach Alberto Lázaro, Julio Gállego-delgado, Julio Osende, Jesús Egido, and Fernando Vivanco A Solubility Optimization Protocol for 2-D Gel Electrophoresis of Cardiac Tissue Brian A. Stanley and Jennifer E. Van Eyk A Method for Effective Depletion of Albumin From Cellular Extracts: Application to Human Myocardium Brian A. Stanley and Jennifer E. Van Eyk Part II. Heart Subproteomes Subcellular Fractionation Mariana E. Guimarães De Araújo and Alfons Huber Subfractionation of Heart Tissue: The "In Sequence" Myofilament Protein Extraction Of Myocardial Tissue Lesley A. Kane, Irina Neverova, and Jennifer E. Van Eyk Optimization of Cardiac Troponin I Pull-Down by Idm Affinity Beads and Seldi-tofT Diane E. Bovenkamp, Brian A. Stanley and Jennifer E.van Eyk Proteomic Analysis Of The Subunit Composition of Complex I (Nadh:Ubiquinone Oxidoreductase) From Bovine Heart Mitochondria Ian M. Fearnley, Joe Carrol and John E. Walker Identification of Targets of Phosphorylation in Heart Mitochondria Roberta A. Gottliebie Part III. Proteomics of Human Atheroma Plaques Characterization of The Human Atheroma Plaque Secretome by Proteomic Analysis Mari-Carmen Durán, Jose L. Martin-Ventura, Sebastián Mas, Maria G. Barderas, Verónica M. Dardé, Ole N. Jensen, Jesús Egido, and Fernando Vivanco Characterization of Hsp-27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome Mari-Carmen Durán, Elisabetta Boeri-Erba, Shabaz Mohammed, Jose L. Martín-Ventura, Jesús Egido, Fernando Vivanco, and Ole N. Jensen Western Array Analysis of Human Atherosclerotic Plaque Wim Martinetar Part IV. The Proteome of Cells of The Cardiovascular System The Proteome of Endothelial Cells Jesús Gonzalez-Cabrero, Mayte Pozo, Mari-Carmen Durán, Rosario de Nicolás, Jesús Egido, and Fernando Vivanco Proteomic Study of Caveolae and Rafts Isolated from Human Endothelial Cells Richard R. Sprenger and Anton J. G. Horrevoets Proteomic Identification of S-nitrosylated Proteins In Endothelial Cells Antonio Martínez-Ruiz and Santiago Lamasgo The Proteome and Secretome of Human Arterial Smooth Muscle Cell Annabelle Dupont and Florence Pinet Real-Time in Vivo Proteomic Identification Of Novel Kinase Substrates in Smooth Muscle Anne A. Wooldridge and Timothy A. Haystead Proteomic Analysis of Vascular Smooth Muscle Cells Treated With Ouabain Alexy V. Pshezhetsky Isolation and Culture of Adult Mouse Cardiac Myocytes Timothy D. O Connell, Manoj C. Rodrigo, and Paul C. Simpson Proteomic Analysis Of Foam Cells Peng-Yuang Yang, Yao-cheng Rui, Peng-Yuan Yang, and Yan-Ling Yuin Isolation of The Platelet Releasate Judith Coppinger, Desmond J. Fitzgerald, and Patricia B. Maguire Enrichment Of Phospho-Tyrosine Proteome Of Human Platelets By Immunoprecipitation Martina Foy, Donald F. Harney, Kieran Wynne, and Patricia B. Maguire Chapter 25 Verónica M. Dardé, Mari-Carmen Durán, Jesús Egido, and Fernando Vivanco Part V. Cardiovascular Biomarkers in Plasma Cardiovascular Biomarkers Discovery By Seldi-tof Mass Spectrometry Olivier Meilhac, Sandrine Delbosc, and Jean-Baptiste Michel Detection of Biomarkers of Stroke Using Seldi-tofe Jean-charles Sanchez, Pierre Lescuyer, Denis Hochstrasser, and Laure Allard Depletion of High-Abundance Proteins In Plasma by Immunoaffinity Substraction For Two-Dimensional Difference Gel Electrophoresis (DIGE) Analysis Verónica M. Dardé, Maria G. Barderas, and Fernando Vivanco A Rapid, Economic and Reproducible Method For Human Serum Delipidation, A

Produktinformationen

Titel: Cardiovascular Proteomics
Untertitel: Methods and Protocols
Editor:
EAN: 9781617376276
ISBN: 978-1-61737-627-6
Format: Kartonierter Einband
Genre: Biologie
Anzahl Seiten: 432
Gewicht: g
Größe: H24mm x B229mm x T152mm
Jahr: 2010
Auflage: Softcover reprint of hardcover 1st ed. 2007.

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