Beschreibung

The first section of this guide provides methods for cardiovascular phenotyping of rodent models; the second section covers bioinformatic integration of phenotypde data with genome-wide genotype and expression data. Chapters include step-by-step protocols.

As two of the leading causes of death worldwide, heart disease and stroke represent a clear target for genomic research aimed at deciphering the genes and cellular pathways that underlie cardiovascular disease and creating improved therapies. In Cardiovascular Genomics: Methods and Protocols , experts in the field provide methods for cardiovascular phenotyping of rodent models in the first section of the volume and for statistical and bioinformatic integration of phenotype data with genome-wide genotype and expression data in the second section. Understanding these diverse methods will allow an individual laboratory to utilize these genomic methods independently or to better prepare for collaboration with scientists having expertise in other disciplines in order to uncover genes affecting cardiovascular disease. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary equipment, materials, and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy-to-use, Cardiovascular Genomics: Methods and Protocols will enable researchers to identify causal genes and novel molecular targets that can lead to vital new treatments for cardiovascular disease.

Inhalt

1. Modes of Defining Atherosclerosis in Mouse Models: Relative Merits and Evolving Standards Alan Daugherty, Hong Lu, Deborah A. Howatt, and Debra L. Rateri 2. Evaluating Micro- and Macro-Vascular Disease, the End Stage of Atherosclerosis, in Rat Models James C. Russell 3. Non-Invasive Blood Pressure Measurement in Mice Minjie Feng and Keith DiPetrillo 4. Direct Blood Pressure Monitoring in Laboratory Rodents via Implantable Radio Telemetry Daniel A. Huetteman and Heather Bogie 5. Measuring Kidney Function in Conscious Mice David L. Mattson 6. Ischemic Stroke in Mice and Rats Aysan Durukan and Turgut Tatlisumak 7. Mouse Surgical Models in Cardiovascular Research Oleg Tarnavski 8. Echocardiographic Examination in Rats and Mice Jing Liu and Dean F. Rigel 9. QTL Mapping in Intercross and Backcross Populations Fei Zou 10. Quantitative Trait Locus Analysis Using J/qtl Randy Smith, Keith Sheppard, Keith DiPetrillo, and Gary Churchill 11. Computing Genetic Imprinting Expressed by Haplotypes Yun Cheng, Arthur Berg, Song Wu, Yao Li, and Rongling Wu 12. Haplotype Association Mapping in Mice Shirng-Wern Tsaih and Ron Korstanje 13. Candidate Gene Association Analysis Jonathan B. Singer 14. Genome-Wide Association Study in Humans J. Gustav Smith and Christopher Newton-Cheh 15. Bioinformatics Analysis of Microarray Data Yunyu Zhang, Joseph Szustakowski, and Martina Schinke 16. eQTL Analysis in Mice and Rats Bruno M. Tesson and Ritsert C. Jansen 17. eQTL Analysis in Humans Lude Franke and Ritsert C. Jansen 18. A Systematic Strategy for the Discovery of Candidate Genes Responsible for Phenotypic Variation Paul Fisher, Harry Noyes, Stephen Kemp, Robert Stevens, and Andrew Brass

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