Capillary Electrophoresis

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This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corr...

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This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras
Inhalt
Part I Principles and Instrumental 1. The CE-Way of Thinking: All is Relative!Philippe Schmitt-Kopplin and Agnes Fekete 2. A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the ElectrophoreticBehaviors of Small Molecules in Free Zone ElectrophoresisPhilippe Schmitt-Kopplin and Agnes Fekete<3. Derivatization in Capillary ElectrophoresisM. Luisa Marina and María Castro-Puyana4. Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MSPeptide Analysis-Strategies and ImplementationMartin Pattky, Katalin Barkovits, Oliver Weiergräber, and Carolin Huhn5. Micellar Electrokinetic Chromatography of AminoglycosidesUlrike Holzgrabe, Stefanie Schmitt, and Frank Wienen6. Microemulsion Electrokinetic ChromatographyWolfgang Buchberger7. Non Aqueous Capillary Electrophoresis Mass SpectrometryChristian W. Klampfl and Markus Himmelsbach8. Ionic Liquids in Capillary ElectrophoresisUlrike Holzgrabe and Joachim Wahl9. CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical ValveFelix J. Kohl and Christian Neusüß10. Capillary Electrophoresis-Inductively Coupled Plasma Mass SpectrometryBernhard Michalke11. Use of CE to Analyze Solutes in Pico- and Nano-Litre Samples from Plant Cells andRhizosphereA. Deri TomosPart II Applications from Small to Macromolecules12. Analysis of Small Ions with Capillary ElectrophoresisJatinder Singh Aulakh, Ramandeep Kaur, and Ashok Kumar Malik13. Metal Ions Analysis with Capillary Zone ElectrophoresisAshok Kumar Malik, Jatinder Singh Aulakh, and Varinder Kaur14. Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis Daniela Fico, Antonio Pennetta, and Giuseppe E. De Benedetto15. Enantiomer Separations by Capillary ElectrophoresisGerhard K. E. Scriba, Henrik Harnisch, and Qingfu Zhu16. Capillary Electrophoresis of Mono- and OligosaccharidesMila Toppazzini, Anna Coslovi, Marco Rossi, Anna Flamigni, Edi Baiutti, and Cristiana Campa17. Use of Capillary Electrophoresis for Polysaccharide Studies and ApplicationsAmelia Gamini, Anna Coslovi, Mila Toppazzini, Isabella Rustighi, Cristiana Campa, Amedeo Vetere,and Sergio Paoletti18. Separation of Peptides by Capillary ElectrophoresisGerhard K. E. Scriba19. Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiaesing Capillary Electrophoresis ApproachPawe Pomastowski, Viorica Railean-Plugaru, and Bogusaw Buszewski20. Capillary Electrophoretic Analysis of Classical Organic PollutantsAshok Kumar Malik, Jatinder Singh Aulakh, and Varinder Kaur21. Capillary Electrophoresis in MetabolomicsTanja Verena Maier and Philippe Schmitt-Kopplin22. Capillary Electrophoresis in Food and FoodomicsClara Ibáñez, Tanize Acunha, Alberto Valdés, Virginia García-Cañas, Alejandro Cifuentes, andCarolina Simó23. Capillary Electrophoresis in Wine ScienceChristian Coelho, Franck Bagala, Régis Gougeon, and Philippe Schmitt-Kopplin