Cancer Epigenetics

This Methods in Molecular Biology(TM) volume reviews epigenetic changes in different tumor types and outlines currently available technologies for detecting epigenetic changes. Includes detailed descriptions and implementation advice for optimal lab results.

The epigenetic regulation plays an important role in normal development and maintenance of tissue specific genes expression in humans and the disturbance of these patterns lead to changes involved in tumor formation. More recently, epigenetic changes have been observed in early stages of tumor development and together with the genetic alterations have been defined as abnormalities, necessary for cancer initiation and progression. In, Cancer Epigenetics: Methods and Protocols , expert researchers reviewed these epigenetics changes in different tumor types and described several technologies that are currently available to detect epigenetic changes. These technologies have lead to a better understanding of the processes in normal and cancerous cells. Written in the highly successful Methods in Molecular Biology ™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Thorough and intuitive, Cancer Epigenetics: Methods and Protocols aids scientists in continuing to study epigenetic alterations used in clinical practice as biomarkers of early cancerous lesions or markers of progression and prognosis.

Includes epigenetic alterations that can be used in clinical practice Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Epigenetic Markers of Early Tumor Development.-Epigenetics of Solid Cancer Stem Cells.-DNA Methylation and Histone Modifications in Breast Cancer.-DNA Methylation Changes in Prostate Cancer.-DNA Methylation in Promoter Region as Biomarkers in Prostate Cancer.-Epigenetics of Bladder Cancer.-Epigenetics in Myeloid Malignancies.-The Epigenetics of Brain Tumors.- DNA Methylation Changes in Cervical Cancers.-Epigenetics of Colon Cancer.-Promoter Methylation in Head and Neck Tumorigenesis.-Epigenome and DNA Methylation in Oral Squamous Cell Carcinoma.-Clinical Implications of Epigenetic Alterations in Human Thoracic Malignancies: Epigenetic Alterations in Lung Cancer.-The Role of microRNAs in the Management of Liver Cancer.- Epigenetics in Ovarian Cancer.- DNA Methylation in Pancreatic Cancer: Protocols for the Isolation of DNA and Bisulfite Modification.-Detection and Analysis of DNA Methylation by Pyrosequencing.-Screening for miRNA Expression Changes using Quantitative PCR (Q-PCR).-Genome-wide Methylation Analysis.-Microarray for Epigenetic Changes: Gene Expression Arrays.-Detecting DNA Methylation using the Methylated CpG Island Amplification and Microarray Technique.- Nutrigenomics Implications for Breast and Colon Cancer Prevention.-Dietary and Lifestyle Factors of DNA Methylation.-Diet, Epigenetics andCancer.-Role of Epigenetics in Cancer Health Disparities.- Multifactorial Etiology of Gastric Cancer.-Epigenetic Epidemiology for Cancer Risk: Harnessing Germline Epigenetic Variation.-Epigenetic Biomarkers in Cancer Epidemiology.