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Calcium Signaling Protocols

  • Kartonierter Einband
  • 360 Seiten
This book explores new protocols and features revised and improved methodologies, making it a valuable reference for all those con... Weiterlesen
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Beschreibung

This book explores new protocols and features revised and improved methodologies, making it a valuable reference for all those contemplating a move into the study of intracellular Ca2+. It features lab-ready procedures and includes key implementation advice.

The regulation of intracellular Ca 2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols , and the developments in the field have also, naturally, continued. With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca 2+ regulation and provided much new information on processes. Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca 2+ signalling for screening, and genetically encoded sensors. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca 2+ .

Klappentext

The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued. With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca2+ regulation and provided much new information on processes. Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca2+ signalling for screening, and genetically encoded sensors. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+.



Inhalt
Part I: General 1. Fluorescent Measurement of [Ca 2+ ] c : Basic Practical Considerations Alec W.M. Simpson 2. Measurement of [Ca 2+ ] i in Whole Cell Suspensions Using Fura-2 Anish Patel, Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert Part II: Specialist Measurement Systems 3. Confocal Microscopy: Theory and Applications for Cellular Signaling Stephen C. Tovey, Paul J. Brighton, Edward T.W. Bampton, Yan Huang, and Gary B. Willars 4. Ratiometric Ca 2+ Measurements Using the FlexStation ® Scanning Fluorometer Ian C.B. Marshall, Izzy Boyfield, and Shaun McNulty 5. Measuring Ca 2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR ® ) Ian C.B. Marshall, Davina E. Owen, and Shaun McNulty 6. Ratiometric [Ca 2+ ] i Measurements in Adherent Cell-lines Using the NOVOstar Microplate Reader Benjamin D. Hunt and David G. Lambert Part III: Measurement of Ca 2+ Channel Activity 7. Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca 2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones Jon Brown, Atticus H. Hainsworth, Alessandro Stefani, and Andrew D. Randall 8. Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells Richard D. Rainbow Part IV: Measurement of Ins(1,4,5)P 3 and Ca 2+ Release Form Intracellular Stores 9. Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [ 3 H]Inositol Labeling Protocols in Permeabilized Cells Alison Skippen, Philip Swigart, and Shamshad Cockcroft 10. Single Cell Imaging Techniques for the Real-Time Detection of IP 3 in Live Cells Carl P. Nelson 11. Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay Darren Smart Part V: Specialist Measurement Techniques 12. Measurement of [Ca 2+ ] i in Smooth Muscle Strips Using Front-Surface Fluorimetry Hideo Kanaide and Katsuya Hirano 13. Calcium Measurements from Whole Heart Using Rhod-2 Bum-Rak Choi 14. Measurement of Changes in Endothelial and Smooth Muscle Ca 2+ in Pressurized Arteries Kim A. Dora and Michael A. Hill 15. Single Cell and Subcellular Measurements of Intracellular Ca 2+ Concentration John G. McCarron, Marnie L. Olson, Susan Chalmers, and John M. Girkin 16. Simultaneous Analysis of Intracellular pH and Ca 2+ from Cell Populations Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch 17. Measurements of Ca 2+ Concentration with Recombinant Targeted Luminescent Probes Denis Ottolini,Tito Calì, and Marisa Brini 18. Chimeric G-Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors Valeria Camarda and Girolamo Calo 19. Compartmentalizing Genetically-Encoded Calcium Sensors David A. Williams, Mastura Monif, and Kate L. Richardson 20. Measurement of Cytosolic-Free Ca 2+ in Plant Tissue Martin R. McAinsh and Carl K.-Y. Ng 21. Measurement of Ca 2+ -ATPase Activity (in PMCA and SERCA1) Danuta Kosk-Kosicka

Produktinformationen

Titel: Calcium Signaling Protocols
Editor:
EAN: 9781493959280
ISBN: 978-1-4939-5928-0
Format: Kartonierter Einband
Genre: Biologie
Anzahl Seiten: 360
Gewicht: 706g
Größe: H254mm x B254mm x T178mm
Jahr: 2016
Auflage: 3. Aufl.