Calcium Binding Proteins in Normal and Transformed Cells

The First European Symposium on Calcium-Binding Proteins in Normal and Transformed Cells was held at the Faculty of Medicine of the "Universit6 Libre de Bruxelles" in Brussels, Belgium, April 20-22, 1989. Delegates from seventeen countries attended. This Symposium was initiated through an EEC Stimulation Program. The formal program included forty verbal presentations by invited speakers and sixty miniposter presentations, and was formulated by the Organizing and Scientific Committee: E. Carmeliet (Leuven), J. P. Collin (Poitiers), S. Forsen (Lund), C. W. Heizmann (Ziirich), D. E. M. Lawson (Cambridge), P. Miroir (Brussels), J. L. Pasteels (Brussels) and R. Pochet (Brussels). This volume contains the papers prepared by the invited speakers. The contributions are grouped according to their general subject matter: Genes of Calcium-Binding Protein Family, Structure/Function Relationships, The Cytoskeleton and Calcium-Binding Proteins, Calcium-Binding Proteins in TransforlIled Cells, Calcium/Lipid-Binding Proteins, Calcium-Binding Proteins Substrates and Immunohistochemistry of Calbindin and Calretinin. The highlights of the symposium are numerous. Among the items to be noted are the growing number of abundant proteins which interact with calcium and sometimes with other second messenger sys tems; specifically pH associated with the tyrosine kinase calpactin, calcYclin, p9Ka induced by growth fac tors, MRP-8 and MRP-14 (also called cystic fibrosis antigen, L1 or calgranulins) forming half the soluble protein of granulocytes. New structure/function relationships on calbindin D9K and calmodulin have emerged from nuclear magnetic resonance and site-directed mutagenesis studies.

Zusammenfassung
Proceedings of the First European Symposium held in Brussels, Belgium, April 20--22, 1989

Inhalt
Calcium and Steady States.- Genes of Calcium Binding Protein Family.- Evolution of the ef-Hand Family of Calcium-Binding Proteins.- Functional Analysis of the Promoter Region of the Gene Encoding Chicken Calbindin-D28K.- Structure of the Human cDNAs and Genes coding for Calbindin-D28K and Calretinin.- Calbindin-D9K (CaBP9K) Gene: a Model for Studying the Genomic Actions of Calcitriols and Calcium in Mammals.- Structure/Function Relationships.- Protein Engineering and Structure/Function Relations in Bovine Calbindin D9k.- Mutant Analysis Approaches to Understanding Calcium Signal Transduction through Calmodulin and Calmodulin Regulated Enzymes.- Parvalbumin, Molecular and Functional Aspects.- Unique Calcium Binding Proteins in Invertebrates.- Structure of the Calcium Release Channel of Skeletal Muscle Sarcoplasmic Reticulum and its Regulation by Calcium.- Structural and Functional Investigation of p68 - a Protein of the Lipocortin/Calpactin Family.- Structure-Function Relations in Troponin C. Chemical Modification Studies.- Ca2 -Dependent Mobility Shift of Parvalbumin in one and two-Dimensional Gel Electrophoresis.- The Cytoskeleton and Calcium Binding Proteins.- Role of Calcium in Secretion and Synthesis in Bovine Adrenal Chromaffin Cells.- Ca2+-Binding Proteins as Component of the Cytoskeleton.- S-100 Proteins: Relationships with Membranes and the Cytoskeleton.- Calcium Binding Proteins In Transformed Cells.- Oncomodulin in Normal and Transformed Cells.- Perturbation of the Calmodulin System in Transformed Cells.- High Levels of oncomodulin and Calmodulin Expression in the Log Phase of Cell Growth in a Chemically Transformed rat fibroblast Cell Line.- Calmodulin and Calbindin in Pancreatic Islet Cells.- Calcium/Lipid-Binding Proteins.- Structural and FunctionalCharacterization of Protein I (p362p112) and II (p32)-Calcium/Phospholipid Binding Proteins with Homologies to Lipocortin I.- Possible Role for two Calcium-Binding Proteins of the S-100 Family, Co-Expressed in Granulocytes and Certain Epithelia.- Phosphorylation of the Calcium-Binding Protein, p68, in the Submembranous Cytoskeleton of Human Placental Syncytiotrophoblast.- Calcyclin-Like Protein from Ehrlich Ascites Tumour Cells - Ca2 - Binding Prpperties, Distribution and Target Protein.- Calcium Binding Proteins Substrates.- Interactions of S-100 Proteins with Proteins Kinases Substrates. Biological implication.- Novel and Selective Inhibitors of CaM-KinaseII and other Calmodulin-Dependent Enzymes.- Structural Details of the Interaction of Calmodulin with the Plasma Membrane Ca2-ATPase.- Erythrocyte Ca2 -ATPase: Activation by Enzyme Oligomerization versus by Calmodulin.- Calpain I Activates Ca2 Transport by the Human Erythrocyte Plama Membrane Calcium Pump.- Inhibitory Regulation by Calcium Ion of Myosin ATPase Activity: Binding of Calcium Ion and Phosphorylation of Myosin.- Immunohistochemistry of Calbindin and Calretinin.- Parvalbumin Expression in Normal and Mutant Xenopus Embryos.- Calretinin and other CaBPs in the Nervous System.- Immunohistochemical Detection of 28KDa Calbindin in Human Tissues.- Comparison Between Rat Brain Calbindin and Calretinin Immunoreactivities.- Contributors.