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Calcium-Binding Proteins and RAGE

  • Kartonierter Einband
  • 424 Seiten
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This Methods in Molecular Biology(TM) book reviews common techniques for studying calcium binding proteins, underlining the diagno... Weiterlesen
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This Methods in Molecular Biology(TM) book reviews common techniques for studying calcium binding proteins, underlining the diagnostic and clinical importance of this family of proteins. Offers materials lists, protocols, troubleshooting advice and more.

A major direction in medical research leading to clinical applications targets the regulation of intracellular calcium and the various human diseases associated with an altered homeostasis of this global second messenger. These diseases include, for example: cardiomyopathy, inflammation, brain disorders, diabetes and cancer. In Calcium-Binding Proteins and RAGE: fr om Structural Basics to Clinical Applications , expert researchers in the field detail many of the methods which are now commonly used to study calcium binding proteins. These methods and techniques, such as calcium-measurements, screening methods, clinical chemistry, and therapy, are generally applicable to many other areas of basic and medical research as well as to diagnostics. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Calcium-Binding Proteins and RAGE: fr om Structural Basics to Clinical Applications underlines the diagnostic and clinical importance of this family of proteins in human diseases and as drug targets.

Part I: Intracellular Ca 2+ - Measurements and Cation Interactions with EF-Hand Proteins 1. Measurement of Intracellular Ca 2+ Concentration in Single Cells using Ratiometric Calcium Dyes Sonal Srikanth and Yousang Gwack 2. Divers Models of Divalent Cation Interaction to Calcium-binding Proteins: Techniques and Anthology Jos A. Cox 3. Probing Ca 2+ Binding Capability of Viral Proteins with the EF-hand Motif by Grafting Approach Yubin Zhou, Shenghui Xue, Yanyi Chen, Jenny J. Yang Part II: Expression, Purification, Biochemical, and Structural Analyses and Cellular Localization 4. New Aspects of Calmodulin-calmodulin Binding Domains Recognition. Emilie Audran, Rania Dagher, Sophie Gioria, Marie-Claude Kilhoffer and Jacques Haiech 5. Purification and Characterization of the Human Cysteine-rich S100A3 Protein and its Pseudo Citrullinated Forms Expressed in Insect Cells Kenji Kizawa, Masaki Unno, Hidenari Takahara, Claus W. Heizmann 6. X-ray Structural Analysis of S100 Proteins Dr. Günter Fritz 7. Purification and Stable Isotope Labeling of the Calcium and Integrin Binding Protein 1 for Structural and Functional NMR Studies Hao Huang and Hans J. Vogel 8. Analysis of Calcium-induced Conformational Changes in Calcium-binding Allergens and Quantitative Determination of their IgE Binding Properties Nuria Parody, Miguel Ángel Fuertes, Carlos Alonso, Yago Pico de Coaña 9. Longistatin, an EF-hand Ca 2+ -binding Protein From Vector Tick: Identification, Purification, and Characterization Anisuzzaman, M. Khyrul Islam, M. Abdul Alim and Naotoshi Tsuji 10. Super-Resolution Microscopy of the Neuronal Calcium-Binding Proteins Calneuron-1 and Caldendrin Johannes Hradsky, Marina Mikhaylova, Anna Karpova, Michael R. Kreutz, and Werner Zuschratter Part III: Interaction of Ca 2+ -Binding Proteins with Targets and Receptors/RAGE 11. NMR Studies of the Interaction of Calmodulin with IQ Motif Peptides Steven M. Damo, Michael D. Feldkamp, Benjamin Chagot, Walter J. Chazin 12. Biochemical and Immunological Detection of Physical Interactions Between Penta-EF-hand Protein ALG-2 and Its Binding Partners Kanae Osugi, Hideki Shibata and Masatoshi Maki 13. Measuring binding of S100 proteins to RAGE by Surface Plasmon Resonance Estelle Leclerc 14. Phage Display Selection of Peptides that Target Calcium-Binding Proteins Stefan W. Vetter Part IV: RAGE-Meditated CELL Signalling and Pathology 15. RAGE-mediated Cell Signalling Ari Rouhiainen, Juha Kuja-Panula, Sarka Tumova, Heikki Rauvala, 16. RAGE Splicing Variants in Mammals Katharina Anna Sterenczak, Ingo Nolte, and Hugo Murua Escobar Part V: Ca 2+ -Binding Proteins in Human Diseases and As Drug Targets 17. Screening for Novel Calcium Binding Proteins that Regulate Cardiac Hypertrophy: CIB1 as an Example Joerg Heineke, MD 18. In Vivo Screening of S100B Inhibitors for Melanoma Therapy Danna B. Zimmer, Rena G. Lapidus and David J. Weber 19. Arrayed Primer Extension Microarray for the Analysis of Genes Associated with Congenital Stationary Night Blindness Kadri Vaidla, Janne Üksti, Christina Zeitz, Eneli Oitmaa 20. Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders Hiroyuki Shimizu and Masatomo Mori Part VI: Cell Line Authentication 21. Human Cell Line Authentication: The Critical First Step in any Project Using Human Cell Lines Robert S. McLaren, Yvonne Reid, and Douglas R. Storts Part VII: Biomarkers, Diagnostic, Clinical Chemistry, and Gene Therapy 22. Detection of Ca 2+ -binding S100 Proteins in Human Saliva by HPLC-ESI-MS Massimo Castagnola, Tiziana Cabras, Federica Iavarone, Chiara Fanali and Irene Messana 23. Clinical use of the Calcium-Binding S100B Protein Ramona Astrand, Johan Undén, and Bertil Romner 24. Sensible use of High-sensitivity Troponin Assays Danielle Hof , Roland Klingenberg, and Arnold von


Titel: Calcium-Binding Proteins and RAGE
Untertitel: From Structural Basics to Clinical Applications
EAN: 9781493959761
ISBN: 978-1-4939-5976-1
Format: Kartonierter Einband
Genre: Biologie
Anzahl Seiten: 424
Gewicht: 832g
Größe: H254mm x B254mm x T178mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2013



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