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Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an onoff switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.
Includes supplementary material: sn.pub/extras
Klappentext
Calcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and signal transduction in cells, to attaching proteins to membranes and serving as an integral source of calcium. In Calcium-Binding Protocols- Volume 1: Reviews and Case Histories and Volume 2: Methods and Techniques-Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant class proteins, and provide a comprehensive collection of proven experimental techniques for studying it both in vitro and in vivo. This second volume focuses on cutting-edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells. The first companion volume, Reviews and Case Histories sets the stage for this volume by introducing the various classes of intra- and extracellular calcium-binding proteins and their mode of action.
Comprehensive and highly practical, the two volumes of Calcium-Binding Protocols provide experimental and clinical biologists with a host of advanced experimental methods that can be applied successfully to the study of both existing and newly discovered members of this critically important class of proteins.
Inhalt
II. Methods to Study Calcium-Binding Proteins Quantitative Analysis of Ca2+ Binding by Flow Dialysis Michio Yazawa Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators Sara Linse Deconvolution of Calcium Binding Curves: Facts and Fiction Jacques Haiech and Marie-Claude Kilhoffer Absorption and Circular Dichroism Spectroscopy Stephen R. Martin and Peter M. Bayley Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins Heinz Fabian and Hans J. Vogel Steady State Fluorescence Spectroscopy Aalim M. Weljie and Hans J. Vogel Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins J. David Johnson and Svetlana B. Tikunova Surface Plasmon Resonance of Calcium-Binding Proteins Karin Julenius Differential Scanning Calorimetry Maria M. Lopez and George I. Makhatadze Isothermal Titration Calorimetry Maria M. Lopez and George I. Makhatadze Multiangle Laser Light Scattering and Sedimentation Equilibrium Leslie D. Hicks, Jean-René Alattia, Mitsuhiko Ikura, and Cyril M. Kay Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in Their Interactions with Regulatory Targets Jill Trewhella and Joanna K. Krueger Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry Amanda L. Doherty-Kirby and Gilles A. Lajoie Synthetic Calcium-Binding Peptides Gary S. Shaw Proteolytic Fragments of Calcium-Binding Proteins Richard D. Brokx and Hans J. Vogel Electron Magnetic Resonance Studies of Calcium-Binding Proteins Lawrence J. Berliner Cadmium-113 and Lead-207 NMR of Calcium-Binding Proteins Teresa E. Clarke and Hans J. Vogel Calcium-43 of NMR ofCalcium-Binding Proteins Torbjörn Drakenberg Exploring Familial Relationships Using Multiple Sequence Alignment Aalim M. Weljie and Jaap Heringa Structure Determination by NMR - Isotope Labeling Monica X. Li, David C. Corson, and Brian D. Sykes Protein Structure Calculation from NMR Data Tapas K. Mal, Stefan Bagby, and Mitsuhiko Ikura Shape and Dynamics of Calcium Binding Proteins Investigated by Nitrogen-15 NMR Relaxation Jörn M. Werner, Iain D. Campbell, and A. Kristina Downing The Use of Dipolar Couplings for the NMR Structure Refinement of a Pair of Calcium Binding EGF Domains Jonathan Boyd, Iain D. Campbell, and A. Kristina Downing Vector Geometry Mapping: A Method to Characterise the Conformation of Helix-Loop-Helix Calcium Binding Proteins Kyoko L. Yap, James B. Ames, Mark B. Swindells, and Mitsuhiko Ikura Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography Ryoji Kobayashi Enzymatic Assays to Compare Calmodulin Isoforms, Mutants and Chimeras Michael P. Walsh, Jacquelyn E. Van Lierop, Cindy Sutherland, Ritsu Kondo, and J. David Johnson Gene Expression in Transfected Cells Kate Hughes, Juha Saarikettu, and Thomas Grundström Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins Anthony Persechini Studying the Spatial Distribution of Ca2+-Binding Proteins: How Does it Work for Calmodulin? Katalin Török, Richard Thorogate, and Steven Howell