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Part I I wasraised in a redbrick Baltimore row housewhere summer was marked by the ti- honored ritual of firefly-chasing a backyard tradition that has endured the gene- tions.Amidtheexcitement,myfatheroftentoldthestoryofhow,whenhewasachild, researchers at the Johns Hopkins University had appealed for the systematic capture of live fireflies en masse. Science had engaged the Baltimore youth in an entrepreneurial quest to jar as many lightning bugs as the dwindling light of dusk would permit. The very next morning, each 100-count glass jar of glowing crawling insects could be exchanged at the University for exactly one crisp dollar bill. Unrecognized at the time by my father, his joyous endeavors had contributed in a profoundwaytotheadvancedmolecularbiologicaltechniquesthatserveasthebasisfor thistextbook.In1947,WilliamMcElroyusedextractsfromthoseveryfirefliestodefine the fundamental reaction underlying the mystical phenomenon of luminescence, and published ''The energy source for bioluminescence in an isolated system'' in the Proceedings of the National Academy of Sciences. Inthedecadessincethatsummer,thestudyandapplicationofbioluminescencehave allowed us to leverage the enduring power of nature's elegance. We have painstakingly harnessedapowerfultoolthatenablesustoseekadeeperunderstandingofthecomplex mechanisms underpinning so many vital biologic systems. This second edition of Methods in Molecular Biology's Bioluminescence: Methods and Protocols serves as a re- ableandutilitariancompilationofthenewestandmostinnovativetechniquesthathave emerged in this rapidly expanding and progressively diverse field. We are indebted to the authors for their thoughtful contributions, inspired by their rigorous dedication to the science of bioluminescence, humbled by the unyielding supportofourcolleagues,andgratefulfortheopportunityprovidedusbyJohnandJan Walker.
Provides an easily accessible reference volume for a wide range of bioluminescence assays and applications written by leading researchers in the field
Comprehensive guide to developing, validating, running, and analyzing bioluminescence assays
Develops detection and imaging methods
Describes the use of a wide variety of bioluminescence tools: various luciferases, reporter gene systems including PIN-G reporters, light emitting bacteria, smart probes, BRET, quantums dots, transgenic animals, etc.
Presents protocols with enough details to be followed and adapted by scientific teams with no previous experience in bioluminescence
Contains numerous notes about potential pitfalls and tips on how to avoid failures
Presents cutting-edge material that will also interest experts in the field of bioluminescence
Provides methods and assays that can be adapted and used in most specialties of biomedical and biotechnological fields: studies of cell trafficking, protein-protein interactions, intracellular signaling, apoptosis, RNAi knockdown, bacterial or viral infections, transplanted cells, stem cells proliferation, vascular flow, and tumors
Covers a wide range of sub-cellular and cell-based assays for in vitro and in vivo analysis as well as non invasive, in vivo, and real time assays and monitoring for animal models
Also includes methods for high throughput analysis of animal models
Includes supplementary material: sn.pub/extras
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