Biological Microarrays

Recent developments in microarray technology have changed the landscape of biology and biomedical research, and they have revolutionized RNA and DNA research. In Biological Microarrays: Methods and Protocols, expert researchers explore exciting new developments in the field, providing a comprehensive approach to biological microarrays that conveys not only the state-of-the-art fundamentals, but also includes applications of the most innovative methods. Chapters address both the application of biological microarrays, including DNA/RNA, apatmer, proteins, tissues, oligonucleotides, carbohydrates, biomaterials, cells, bacteria, and virus microarrays, and also explore the different techniques used for generating microarray platforms. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and revolutionary, Biological Microarrays: Methods and Protocols serves as a primary source for academics, practitioners, and professionals in related fields, including biologists, biotechnologists, biochemists, analytical chemists, and biomedical, physical, and microsystems engineers, to name a few, appealing to all of those interested in the present and future state of biological microarray research.

Comprehensive presentation of various biological microarrays and their applications Includes methods for analysis and characterization of the resulting arrays Provides description of synthesis methods and areas of use Includes supplementary material: sn.pub/extras

Inhalt
RNA and DNA Microarrays.- Aptamer Arrays.- Oligonucleotide microarrays for identification of microbial pathogens and detection of their virulence-associated or drug-resistance determinants.- Protein MicroArrays printed from DNA MicroArrays.- Lithographically-defined two- and three-dimensional tissue microarrays.- Ratiometric lectin microarray analysis of the mammalian cell surface glycome.- Cell microarrays based on hydrogel microstructures for the application to cell-based biosensor.- Fabrication of Bacteria and Virus Microarrays Based on Polymeric Capillary Force Lithography.- 3D Polymer Scaffold Arrays.- PDMS microfluidic capillary systems for patterning proteins on surfaces and performing miniaturized immunoassays.- Merging Photolithography and Robotic Protein Printing to Create Cellular Microarrays.- Generation of Protein and Cell Microarrays on Functionalized Surfaces.- Microprinting of Liver Micro-organ for Drug Metabolism Study.- Microcontact printing.- Micromolding for the Fabrication of Biological Microarrays.- Progress Report on Microstructured Surfaces Based on Chemical Vapor Deposition.- Methods for Forming Human Microvascular Tubes in Vitro and Measuring Their Macromolecular Permeability.- Microarray Bioinformatics.