Bacterial Therapy of Cancer

This volume explores the evolution of bacterial cancer therapy and describes the modern techniques used in therapy today. The chapters in this book cover a broad range of topics such as the development of tumor-targeting Salmonella typhimurium A1-R, a microfluidic device for precise quantification of the interactions between tumor-targeting bacteria and tumor tissue, non-invasive in vivo imaging of bacteria-mediated cancer therapy using bio-luminescent bacteria, methods to achieve remote-control of therapeutic gene expression in tumor-targeting bacteria, and cell-cycle decoy of cancer cells resistant to cytotoxic drugs to drug sensitivity by S. typhimurium A1-R. This book concludes with a chapter on the future potential of bacterial therapy of cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and informative, Bacterial Therapy of Cancer: Methods and Protocols is a valuable resource for anyone who is interested in cancer and bacterial therapy.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Autorentext
Robert M. Hoffman, Ph.D. Professor of Surgery, University of California, San Diego President, AntiCancer, Inc. 7917 Ostrow Street San Diego, CA 92111 USA TEL: 1-858-654-2555 FAX: 1-858-268-4175 Email: all@anticancer.com

Inhalt
Tumor Targeting Salmonella Typhimurium A1-R: An Overview.- Enhancement of Tumor-Targeted Delivery of Bacteria with Nitroglycerin Involving Augmentation of the ERP Effect.- Oral Delivery of Tumor-Targeting Salmonella to Treat Cancer in Mice.- Microfluidic Device to Quantify the Behavior of Therapeutic Bacteria in Three-Dimensional Tumor Tissue.- Tumor-Targeting Therapy Using Gene-Engineered Anaerobic-Nonpathogenic Bifidobacterium Longum.- Noninvasive In Vivo Imaging to Follow Bacteria Engaged in Cancer Therapy.- In Vivo Bioluminescence Imaging of Intra-Tumoral Bacteria.- Employment of Salmonella in Cancer Gene Therapy.- Development of a Targeted Gene Delivery System Using Escherichia Coli.- Isolation and Analysis of Suppressor Mutations in Tumor-Targeted msbB Salmonella.- Determination of Plasmid Segregational Stability in a Growing Bacterial Population.- Visualization of Anti-Cancer Salmonella Typhimurium Engineered for Remote-Control of Therapeutic Proteins.- Methods for Tumor Targeting with Salmonella Typhimurium A1-R.- Salmonella Typhimurium A1-R and Cell-Cycle Decoy Therapy of Cancer.- Future of Bacterial Therapy of Cancer.