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Avian Influenza Virus

  • Kartonierter Einband
  • 142 Seiten
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This exciting and cutting-edge book examines the fundamental virological methods for avian influenza virus research and diagnostics. It covers some of the newest molecular procedures currently used for basic and applied research.

The avian influenza (AI) virus, which is the type A influenza virus adapted to an avian host, has been an important pathogen for the poultry industry worldwide for many years. Although the cause was unknown at the time, one of the first recognized outbreaks of highly pathogenic AI (HPAI) virus occurred in Europe in the 1870s. Since 1955, when the AI virus was first identified, there have been about 24 outbreaks of the HPAI virus in domestic poultry worldwide. Of those, perhaps the most consequential HPAI virus strain to emerge has been the Asian H5N1 HPAI virus, which was first seen in Southeast Asia around 1997. Until sometime in 2003, this strain had been mainly involved in sporadic outbreaks and was relatively contained. Then in 2004, reports of infection in poultry increased throughout Asia at about the same time as fatal cases of human infections in several Southeast Asian countries were confirmed. By 2006, the virus had spread further throughout Asia, into Africa and Europe, and as far west as the United Kingdom.

Contains protocols for virus detection, isolation and propagation, and virus characterization

Includes cellular immunological methods and immunohistochemistry with a focus on avian hosts

Covers reverse genetics with avian influenza virus

Details wild bird monitoring including sampling methods and virus detection

Includes supplementary material: sn.pub/extras


With the growing global fear of a major pandemic, avian influenza virus research has increased greatly in importance during this young century. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental yet essential virological methods for AI virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research.  Exciting, cutting-edge new methods focus on studying the virus itself and work with avian hosts, an area greatly lacking in research.  Following the format of the highly successful Methods in Molecular Biology™ series, each chapter provides readily reproducible laboratory protocols offering step-by-step instruction and lists of the necessary equipment for the task.

Comprehensive and timely, Avian Influenza Virus equips diagnosticians and researchers with the current tools and information they need to learn more about this high impact disease.

Avian Influenza Virus Table of Contents 1. A brief introduction to avian influenza virus Erica Spackman 2. Avian Influenza Virus Sample Types, Collection, and Handling Mary-Lea Killian 3. Avian influenza virus RNA extraction from tissue and swab material Erica Spackman and David L. Suarez 4. Type-A Influenza Virus Detection and Quantitation by Real-time RT-PCR Erica Spackman and David L. Suarez 5. Detection and Identification of the H5 Hemagglutinin subtype by Real-time RT-PCR Erica Spackman and David L. Suarez 6. Avian influenza virus isolation and propagation in chicken eggs Peter R. Woolcock 7. Hemagglutination Assay for Avian Influenza Virus Mary-Lea Killian 8. Hemagglutination-Inhibition Test for Avian Influenza Virus Subtype Identification and the Detection and Quantititation of Serum Antibodies to Avian Influenza Virus Janice C. Pedersen 9. Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Subtype or Neuraminidase Antibody Specificity Janice C. Pedersen 10. Immunohistochemical Staining for the Detection of Avian Influenza Virus in Tissues Mary Pantin-Jackwood 11. Wild bird Surveillance for Avian Influenza Virus Justin D. Brown and David E. Stallknecht 12. Reverse Genetics of Avian Influenza Virus Chang-Won Lee and David L. Suarez 13. Evaluating the Cell Mediated Immune Response of Avian Species to Avian Influenza Viruses Darrell R. Kapczynski 14. Measurment of avian cytokines with real-time RT-PCR following infection with avian influenza virus Darrell R. Kapczynski and Michael H. Kogut


Titel: Avian Influenza Virus
EAN: 9781617378508
ISBN: 978-1-61737-850-8
Format: Kartonierter Einband
Herausgeber: Springer, Berlin
Genre: Medizin
Anzahl Seiten: 142
Größe: H235mm x B235mm x T155mm
Jahr: 2010
Untertitel: Englisch
Auflage: Softcover reprint of hardcover 1st ed. 2008

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