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Atomic Force Microscopy in Biomedical Research

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Atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Covering topics like molecule imaging,... Weiterlesen
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Beschreibung

Atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Covering topics like molecule imaging, nanoscale surface analysis and cellular imaging, this volume features the most up-to-date techniques currently in use.

With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use. Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.

Applicable to a wide variety of fields and disciplines

Features a comprehensive collection of practical applications and techniques

Includes tips from the experts as well as key implementation advice



Inhalt

Part I: The Basics of Atomic Force Microscopy

1. How the Atomic Force Microscope Works

Bruno Torre, Davide Ricci, and Pier Carlo Braga

2. Measurement Methods in Atomic Force Microscopy

Bruno Torre, Claudio Canale, Davide Ricci, and Pier Carlo Braga

3. Recognizing and Avoiding Artifacts in Atomic Force Microscopy Imaging

Claudio Canale, Bruno Torre, Davide Ricci, and Pier Carlo Braga

Part II: Molecule Imaging

4. Imaging the Spatial Orientation of Subunits within Membrane Receptors by Atomic Force Microscopy

Stewart M Carnally, J. Michael Edwardson, and Nelson P. Barrera

5. High Resolution Imaging of Immunoglobulin G (IgG) Antibodies and Other Biomolecules Using Amplitude Modulation Atomic Force Microscopy in Air

Sergio Santos and Neil H. Thomson

6. Atomic Force Microscopy of Ex vivo Amyloid Fibrils

Claudio Canale, Annalisa Relini, and Alessandra Gliozzi

7. Studying Collagen Self-Assembly by Time-Lapse High-Resolution Atomic Force Microscopy

Clemens M. Franz and Daniel J. Muller

8. Atomic Force Microscopy Imaging of Human Metaphase Chromosomes in Liquid

Osamu Hoshi and Tatsuo Ushiki

9. Atomic Force Microscopy of Proteasome Assemblies

Maria Gaczynska and Pawel A. Osmulski

10. Atomic Force Microscopy of Isolated Mitochondria

Bradley E. Layton and M. Brent Boyd

11. Imaging Native Membrane Proteins Using the Atomic Force Microscope

Andreas Engel

Part III: Nanoscale Surface Analysis and Cell Imaging

12. Atomic Force Microscopy Investigation of Viruses

Alexander McPherson and Yurii G. Kuznetsov

13. Determination of the Kinetic On and -Off Rate of Single Virus-Cell Interactions

Christian Rankl, Linda Wildling, Isabel Neundlinger, Ferry Kienberger, Hermann Gruber, Dieter Blaas, and Peter Hinterdorfer

14. Atomic Force Microscopy as a Tool for the Study of the Ultrastructure of Trypanosomatid Parasites

Wanderley de Souza, Gustavo M. Rocha, Kildare Miranda, Paulo M. Bisch, and Gilberto Weissmuller

15. Normal and Pathological Erythrocytes Studied by Atomic Force Microscopy

Andreas Ebner, Hermann Schillers, and Peter Hinterdorfer

16. The Growth Cones of Living Neurons Probed by the Atomic Force Microscope

Davide Ricci, Massimo Grattarola, and Mariateresa Tedesco

17. Highlights on Ultrastructural Pathology of Human Sperm

Narahari V. Joshi, Ibis Cruz, and Jesus A. Osuna

18. High-speed Atomic Force Microscopy and Biomolecular Processes

Takayuki Uchihashi and Toshio Ando

Part IV: Non-Topographical Applications (Force-Spectroscopy)

19. Atomic Force Microscopy in Mechanobiology: Measuring Micro-Elastic Heterogeneity of Living Cells

Evren U. Azeloglu and Kevin D. Costa

20. Force-Clamp Measurements of Receptor-Ligand Interactions

Félix Rico, Calvin Chu, and Vincent T. Moy

21. Measuring Cell Adhesion Forces: Theory and Principles

Martin Benoit and Christine Selhuber-Unkel

22. Nanoscale Investigation on E. coli Adhesion to Modified Silicone Surfaces

Ting Cao, Haiying Tang, Xuemei Liang, Anfeng Wang, Gregory W. Auner, Steven O. Salley, and K.Y. Simon Ng

Part V: Investigating Drug Action

23. Imaging Bacterial Shape, Surface, and Appendages Before and After Treatment with Antibiotics

Pier Carlo Braga and Davide Ricci

24. Thymol-Induced Alterations in Candida albicans Imaged by Atomic Force Microscopy

Pier Carlo Braga and Davide Ricci

25. Atomic Force Microscope-Enabled Studies of Integrin-Extracellular Matrix Interactions in Vascular Smooth Muscle and Endothelial Cells

Zhe Sun and Gerald A. Meininger

26. Atomic Force Microscopy Studies on Circular DNA Structural Changes by Vincristine and Aspirin

Zhongdang Xiao, Lili Cao, Dan Zhu, and Zuhong Lu

Part VI: Atomic Force Microscopy as a Nanotool

27. Combined Atomic Force Microscopy and Fluorescence Microscopy

Miklós S.Z. Kellermayer

28. Chemical Modifications of Atomic Force Microscopy Tips

Régis Barattin and Normand Voyer

29. Atomic Force Microscopy as Nanorobot

Ning Xi, Carmen Kar Man Fung, Ruiguo Yang, King Wai Chiu Lai, Donna H. Wang, Kristina Seiffert-Sinha, Animesh A. Sinha, Guangyong Li, and Lianqing Liu

Produktinformationen

Titel: Atomic Force Microscopy in Biomedical Research
Untertitel: Methods and Protocols
Editor:
EAN: 9781493962792
ISBN: 1493962795
Format: Kartonierter Einband
Herausgeber: Humana Press
Genre: Medizin
Anzahl Seiten: 524
Gewicht: 974g
Größe: H254mm x B178mm x T28mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2011

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