Apoptosis

This volume presents the techniques to investigate the critical steps involved in the apoptotic process. Coverage includes detection of apoptosis, modifications of apoptotic proteins during apoptosis, and analysis of apoptosis in different organs.

The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition , expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular Biology ™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.

Provides an easily accessible reference volume for methods to investigate the critical steps in apoptotic cell death written by leading researchers in the field Details the process of how to induce, measure and differentiate apoptosis in various models Covers a wide range of model systems for apoptosis detection from cell-based assays to measurements at the level of organ or organism Presents a modular approach to experimental design, covering model biological systems, screening modalities, detection technologies, approaches to data interpretation, and tips for troubleshooting Comprehensive guide to methodologies applied to elucidate the molecular mechanisms of apoptosis Includes supplementary material: sn.pub/extras

Inhalt
Detection of Apoptosis.- Analysing Caspase Activation and Caspase Activity in Apoptotic Cells.- Flow Cytometry-Based Apoptosis Detection.- Live to Dead Cell Imaging.- Detection of Apoptosis in Tissue Sections.- Detection of Apoptosis in Cell-Free Systems.- Detection of Non-Apoptotic Cell Death.- Methods to Analyze Cellular Necroptosis.- Detection of Cell Death by Autophagy.- Modifications of Apoptotic Proteins during Apoptosis.- Methods to Analyze Transglutamination of Proteins Involved in Apoptosis.- Methods to Analyze S-nitrosylation of Proteins Involved in Apoptosis.- Application of In Vivo EPR for Tissue pO2 and Redox Measurements.- Analysis of the Function of Major Regulators of Apoptosis/Cell Survival.- Assays to Measure p53-Dependent and -Independent Apoptosis.- Measurement of Changes in Cdk2 and Cyclin O-Associated Kinase Activity in Apoptosis.- Fluorometric Methods for Detection of Mitochondrial Membrane Permeabilization in Apoptosis.- Regulation of Apoptosis by the Unfolded Protein Response.- Detection of Uncoupling Protein-2 (UCP2) As a Mitochondrial Modulator of Apoptosis.- Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis.- Assessment of Apoptotic Cell Phagocytosis by Macrophages.- Analysis of Apoptosis in Different Organs.- Detection of Apoptosis in Mammalian Development.- Detection of Apoptosis in the Central Nervous System.- Genetic Mapping of Anti-Apoptosis Pathways in Myeloid Progenitor Cells.- Analysis of Apoptosis in Isolated Primary Cardiac Myocytes.- Cell Death in Myoblasts and Muscles.- Analysis of Apoptosis in Model Organisms.- Reliable Method for Detection of Programmed Cell Death in Yeast.- Detection of Cell Death in Drosophila.- Detecting Apoptotic Cells and Monitoring Their Clearance in the Nematode Caenorhabditis elegans.- Detection of Herpes Simplex Virus Dependent Apoptosis.