Apoptosis and Cancer

The aim of this book is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use. Tricks of the trade and notes on troubleshooting and avoiding known pitfalls are included. The book is aimed at cancer researchers, molecular and cellular biologists, and biochemists.

Readily reproducible, cutting-edge techniques for studying the biology of apoptosis Step-by-step instructions that ensure successful results Tricks of the trade and notes on troubleshooting and avoiding known pitfalls

Inhalt
Preface.- Contributors.- Modulation of Apoptosis to Reverse ChemoresistanceGil Mor, Michele K. Montagna, and Ayesha B. Alvero.- Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-Induced ApoptosisSuparna Mazumder, Dragos Plesca, and Alexandru Almasan.- Flow Cytometry Enumeration of Apoptotic Cancer Cells by Apoptotic RateDavid Diaz, Alfredo Prieto, Eduardo Reyes, Hugo Barcenilla, Jorge Monserrat, and Melchor Alvarez-Mon.- Detection of Cancer-Related Proteins in Fresh-Frozen Ovarian Cancer Samples Using Laser Capture MicrodissectionDan-Arin Silasi, Ayesha B. Alvero, Jechiel Mor, Rui Chen, Han-Hsuan Fu, Michele K. Montagna, and Gil Mor.- Flow Cytometric Detection of Activated Caspase-3Richard Fox and Martine Aubert.- The ChemoFx™ Assay: An Ex Vivo Chemosensitivity and Resistance Assay for Predicting Patient Response to Cancer ChemotherapyStacey L. Brower, Jeffrey E. Fensterer, and Jason E. Bush.- Correlation of Caspase Activity and In Vitro Chemo-Response in Epithelial Ovarian Cancer Cell LinesAyesha B. Alvero, Michele K. Montagna, and Gil Mor.- Assessing Expression of Apoptotic Markers Using Large Cohort Tissue MicroarraysElah Pick, Mary M. McCarthy, and Harriet M. Kluger.- Mitochondria Potential, Bax Activation, and Programmed Cell DeathC. Michael Knudson and Nicholas M. Brown.- In Vitro and In Vivo Apoptosis Detection Using Membrane Permeant Fluorescent-Labeled Inhibitors of CaspasesBrian W. Lee, Michael R. Olin, Gary L. Johnson, and Robert J. Griffin.- Caspase Activity AssaysAndrew L. Niles, Richard A. Moravec, and Terry L. Riss.- Multiplex Caspase Activity and Cytotoxicity AssaysAndrew L. Niles, Richard A. Moravec, and Terry L. Riss.- Homogeneous, Bioluminescent Proteasome AssaysMartha A. O'Brien, Richard A. Moravec, Terry L. Riss, andRobert F. Bulleit.- A Simple Method for Profiling miRNA ExpressionJia-Wang Wang and Jin Q. Cheng.- Apoptotic Caspase Activation and ActivityJean-Bernard Denault and Guy S. Salvesen.- Biochemical Analysis of the Native TRAIL Death-Inducing Signaling ComplexHenning Walczak and Tobias L. Haas.- Laser Microdissection Sample Preparation for RNA AnalysesChristopher J. Vega.