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Antiviral RNAi

  • Kartonierter Einband
  • 428 Seiten
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Here is a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for develop... Weiterlesen
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Here is a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for development and optimization of RNAi-based antiviral drugs. The methods here can also be applied outside the context of a virus infection.

Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs of viral or cellular origin affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Part I: General Introduction 1. Defense and counter-defense in the RNAi-based antiviral immune system in insects Joël T. van Mierlo, Koen W.R. van Cleef, and Ronald P. van Rij 2. RNAi and cellular miRNAs in infections by mammalian virusesJoost Haasnoot and Ben Berkhout 3. Viral miRNAs Karlie Plaisance-Bonstaff, and Rolf Renne 4. Progress in RNAi-based antiviral therapeutics Jiehua Zhou and John J. Rossi 5. Chemical modification of small interfering RNA Jesper B. Bramsen and Jørgen Kjems Part II: Viral small RNAs 6. Viral small RNA cloning and sequencing Valérie Gausson and Maria-Carla Saleh 7. Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets Christophe Antoniewski 8. Computational prediction of viral miRNAs Adam Grundhoff 9. Detection of Viral microRNAs by Northern Blot Analysis Lydia V. McClure, Yao-Tang Lin, and Christopher S. Sullivan 10. Detection of viral microRNA with S1 Nuclease Protection assay Matthias John and Sébastien Pfeffer 11. Characterization of RISC associated adenoviral small RNAs Ning Xu and Göran Akusjärvi Part III: RNAi-based antiviral defense 12. Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell CultureKoen W.R. van Cleef, Joël T van Mierlo, Marius van den Beek, and Ronald P. van Rij 13. Dicer assay in Drosophila S2 cell extract Baojun Yang and Hongwei Li 14. Slicer activity in Drosophila melanogaster S2 extract Arabinda Nayak and Raul Andino 15. Gel Mobility Shift Assays for RNA-binding viral RNAi suppressors Tibor Csorba and József Burgyán 16. dsRNA uptake in adult Drosophila Benjamin Obadia and Maria-Carla Saleh Part IV: Antiviral RNAi therapy 17. Design of small interfering RNAs for antiviral applications Diana Rothe, Erik J. Wade, and Jens Kurreck 18. RNAi inducing lentiviral vectors for anti-HIV-1 gene therapy Ying Poi Liu, Jan-Tinus Westerink, Olivier ter Brake, and Ben Berkhout 19. Production of multicopy shRNA lentiviral vectors for antiviral therapy Scot D. Henry, Quiwei Pan, and Luc J.W. van der Laan 20. Intranasal delivery of antiviral siRNA Sailen Barik 21. Antibody mediated delivery of siRNAs for anti-HIV therapy Sang-Soo Kim, Sandesh Subramanya, Dan Peer, Motomu Shimaoka, and Premlata Shankar 22. Aptamer-targeted RNAi for HIV-1 therapy Jiehua Zhou and John J. Rossi Part V: RNAi screens to study virus-host interactions 23. RNAi screening for host factors involved in viral infection using Drosophila cells Sara Cherry 24. Genome-wide RNAi Screen for Viral Replication in Mammalian Cell Culture Bhupesh K. Prusty, Alexander Karlas, Thomas F. Meyer, and Thomas Rudel 25. RNAi screening in mammalian cells to identify novel host cell molecules involved in the regulation of viral infections Carolyn B. Coyne and Sara Cherry


Titel: Antiviral RNAi
Untertitel: Concepts, Methods, and Applications
EAN: 9781493958252
ISBN: 1493958259
Format: Kartonierter Einband
Herausgeber: Humana Press
Genre: Medizin
Anzahl Seiten: 428
Gewicht: 800g
Größe: H254mm x B178mm x T22mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2011

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