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Amyloid Proteins

  • Kartonierter Einband
  • 547 Seiten
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This third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that... Weiterlesen
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This third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that were not addressed in the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro assays that focus on a variety of amyloids and how to study these peptides and proteins. Part Two describes cell culture models and assays, and Part Three explores methods on how to extract amyloid from tissue, its detection, and its characterization in vitro or in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Amyloid Proteins: Methods and Protocols, Third Edition is a valuable resource for both students and scientists who are new to the field, as well as experienced researchers who would like to progress their research with the latest available techniques.


Preface...Table of Contents...Contributing Authors...Part I: In Vitro Models and Assays1. Preparation of Pure Populations of Amyloid-ß Protein Oligomers of Defined Size Eric Y. Hayden, Joseph L. Conovaloff, Ashley Mason, Gal Bitan, and David B. Teplow 2. Preparation of Well-Defined and Stable ß-Barrel Pore-Forming Amyloid-ß42 Oligomers Montserrat Serra-Batiste, Martí Ninot-Pedrosa, Eduard Puig, Sonia Ciudad, Margarida Gairí, and Natàlia Carulla3. Unveiling Brain Aß Heterogeneity through Targeted Proteomic AnalysisAgueda Rostagno, Thomas A. Neubert, and Jorge Ghiso4. Preparation of a-Synuclein Amyloid Assemblies for Toxicity Experiments Serene W. Chen and Nunilo Cremades 5. Generation and Characterization of Stable a-Synuclein OligomersLeire Almandoz-Gil, Martin Ingelsson, and Joakim Bergström6. In Vitro Analysis of a-Synuclein Amyloid Formation and Cross-ReactivityIstvan Horvath, Sandra Rocha, and Pernilla Wittung-Stafshede7. Preparation of Tau Oligomers after Protein Extraction from Bacteria and Brain Cortices Elentina K. Argyrousi, Agnieszka Staniszewski, Russell E. Nicholls, and Ottavio Arancio8. Purification and Characterization of Low-n Tau Oligomers Senthilvelrajan Kaniyappan, Ram Reddy Chandupatla, and Eckhard Mandelkow9. Preparation and Characterization of Tau Oligomer Strains Urmi Sengupta, Mariana Carretero-Murillo, and Rakez Kayed10. Purification and Fibrillation of Recombinant Human Amyloid-ß, Prion Protein and Tau under Native ConditionsAlexander Sandberg and Sofie Nyström11. Preparation of Amyloidogenic Aggregates from EF-Hand ß -parvalbumin and S100 ProteinsJavier Martínez, Joana S. Cristóvão, Rosa Sánchez, Maria Gasset , and Cláudio M. Gomes12. Kinetic Analysis of Amyloid FormationGeorg Meisl, Thomas C.T. Michaels, Sara Linse, and Tuomas P. J. Knowles13. Mapping Amyloid Regions in Gad m 1 with Peptide Arrays Rosa Sánchez, Javier Martínez, Laura Montoya, Milagros Castellanos, and Maria Gasset14. Non-Invasive Structural Analysis of Intermediate Species during Fibrillation: An Application of Small-Angle X-Ray ScatteringAnnette Eva Langkilde, Fátima Herranz-Trillo, Pau Bernadó, and Bente Vestergaard15. Analysis of Covalent Modifications of Amyloidogenic Proteins using Two-Dimensional Electrophoresis: Prion Protein and Its SialylationElizaveta Katorcha and Ilia V. Baskakov16. Amplification and Detection of Minuscule Amounts of Misfolded Prion Protein by Using The Real-Time Quaking-Induced-ConversionMatthias Schmitz, Niccolò Candelise, Franc Llorens, and Inga ZerrPart II: Cell Culture Models and Assays17. Bacterial AmyloidsMargery L. Evans, Elizabeth Gichana, Yizhou Zhou, and Matthew R. Chapman18. Addressing Intracellular Amyloidosis in Bacteria with RepA-WH1, a Prion-Like ProteinLaura Molina-García, Fátima Gasset-Rosa, María Moreno-del Álamo, Susana Moreno-Díaz de la Espina, and Rafael Giraldo19. Study of Amyloids Using YeastReed B. Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey, and Herman K. Edskes20. Neurotoxic Ca2+ Signaling Induced by Amyloid-ß Oligomers in Aged Hippocampal Neurons In VitroLucía Núñez, María Calvo-Rodríguez, Erica Caballero, Mónica García-Durillo, and Carlos Villalobos 21. Preparation and Culturing of Human Primary Vascular CellsFinnbogi R. Thormodsson, Ingvar H. Olafsson, and Dadi Th. Vilhjalmsson22. Live Imaging of Pathological Tau Protein and Tau Antibodies in a Human Neuron-Like Cellular Model Dov B. Shamir, Yan Deng, and Einar M. Sigurdsson23. Effects of Amyloid-ß Peptide on the Biology of Human Neural Stem CellsAdela Bernabeu-Zornoza, Raquel Coronel, María Lachgar, Charlotte Palmer, and Isabel ListePart III: In Vivo Models and Assays24. Development of Mouse Monoclonal Antibodies against Human Amyloid Fibril Proteins for Diagnostic and Research PurposesGunilla T. Westermark, Elisabet Ihse, and Per Westermark25. Identification and Characterization of Amyloid-ß Accumulation in Synaptic MitochondriaShi Fang Yan, Firoz Akhter, Alexander A. Sosunov, and Shirley ShiDu Yan26. Biochemical Properties of Pathology-Related Tau Species in Tauopathy Brains: An Extraction Protocol for Tau Oligomers and AggregatesNaruhiko Sahara and Taeko Kimura27. Tau Assembly into FilamentsMar Pérez, Raquel Cuadros, and Miguel Medina28. Quantitative Metabolomics in Alzheimer s Disease: Technical Considerations for Improved ReproducibilitySergio Veiga, Judith Wahrheit, Andrés Rodríguez-Martín, and Denise Sonntag 29. Detecting Circulating MicroRNAs as Biomarkers in Alzheimer's DiseaseAidan Kenny, Eva M. Jimenez-Mateos, Miguel Calero, Miguel Medina, and Tobias Engel30. Luminescent Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues K. Peter R. Nilsson, Mikael Lindgren, and Per Hammarström31. Characterization of Amyloid-ß Plaques and Autofluorescent Lipofuscin Aggregates in Alzheimer s Disease Brain: A Confocal Microscopy ApproachAlejandra Kun, Fernando González-Camacho, Silvia Hernández, Alexandra Moreno-García, Olga Calero, and Miguel Calero32. In Vivo Imaging of Tauopathy in MiceSenthilkumar Krishnaswamy, Qian Wu, Yan Lin, Wajitha J. Rajamohamedsait, Hameetha B. Rajamohamedsait, and Einar M. Sigurdsson33. In Vivo Evaluation of Neuronal Transport in Murine Models of Neurodegeneration using Manganese-Enhanced MRIAnne Bertrand, Maria Baron, Dung M. Hoang, Lindsay K. Hill, Sebastian L. Mendoza, Einar M. Sigurdsson, and Youssef Z. Wadghiri


Titel: Amyloid Proteins
Untertitel: Methods and Protocols
EAN: 9781493992935
ISBN: 978-1-4939-9293-5
Format: Kartonierter Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 547
Gewicht: 1300g
Größe: H24mm x B251mm x T185mm
Jahr: 2019
Auflage: 3. Aufl.

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