Alcohol

Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics.

Step-by-step protocols for the most commonly used models of acute and chronic ethanol exposure Detailed procedures for cell culture experiments with ethanol Comprehensive methods for studies of fetal alcohol syndrome (FAS) including Zebrafish, avian embryo and rodent models Up-to-date proteomics and micro-array protocols Specific protocols of interest to neuroscientists, immunologists, physiologists, cell biologists and biochemists Includes supplementary material: sn.pub/extras

Inhalt
Animal Models of Acute and Chronic Ethanol Exposure.- Acute Models of Ethanol Exposure to Mice.- A Voluntary Oral-feeding Rat Model for Pathological Alcoholic Liver Injury.- Intragastric Ethanol Infusion Model in Rodents.- A Practical Method of Chronic Ethanol Administration in Mice.- Animal Models of Ethanol Exposure During Development.- Analysis of Ethanol Developmental Toxicity in Zebrafish.- The Avian Embryo in Fetal Alcohol Research.- Artificial Rearing.- Intragastric Intubation of Alcohol During the Perinatal Period.- Cell Culture Approaches to Studying Ethanol Exposure.- Human Monocytes, Macrophages, and Dendritic Cells: Alcohol Treatment Methods.- Generation and Use of Primary Rat Cultures for Studies of the Effects of Ethanol.- Development and Properties of HepG2 Cells That Constitutively Express CYP2E1.- Modeling the Impact of Alcohol on Cortical Development in a Dish: Strategies from Mapping Neural Stem Cell Fate.- Regulation of Specific Organ Systems in Response to Ethanol.- Acetaldehyde-induced Barrier Disruption and Paracellular Permeability in Caco-2 Cell Monolayer.- Alcohol-Induced Oxidative Stress in the Liver: In Vivo Measurements.- Isolation of Kupffer Cells from Rats Fed Chronic Ethanol.- Dendritic Cells in Chronic In Vivo Ethanol Exposure Models.- Formation and Immunological Properties of Aldehyde-derived Protein Adducts following Alcohol Consumption.- Assessment of Natural Killer (NK) and NKT Cells in Murine Spleens and Livers.- Polyclonal and Antigen-specific Responses of T Cells and T Cell Subsets.- B-Cell Studies in Chronic Ethanol Mice.- Histological Analysis of Bone.- Assessing Effects of Alcohol Consumption on Protein Synthesis in Striated Muscles.- Methods to Investigate the Effects of Chronic Ethanol on Adipocytes.- Proteomic and Genomic Approaches to Study Response to Ethanol.- Proteomic Approaches to Identify and Characterize Alterations to the Mitochondrial Proteome in Alcoholic Liver Disease.- Alcoholic Liver Disease and the Mitochondrial Ribosome: Methods of Analysis.- Microarray Analysis of Ethanol-Induced Changes in Gene Expression.