Advances in Lactoferrin Research

Following the two meetings on Lactoferrin Structure and Function that were held in Honolulu, Hawaii, in 1993 and 1995, the Third International Conference on Lactoferrin Structure and Function was held in Le Touquet, France, and has successfully reinforced and diversified the previously created bridges between biochemists, clinicians, and companies. In fact, scientists, physicians, and people of industry from different domains have brought a wealth of recent information concerning biochemistry and technical advances in the identification of lactoferrin-derived compounds as well as cell biology, molecular biol ogy, pathology, and medical applications of lactoferrin and lactoferrin-derived com pounds. We were so delighted with the rapid growth of knowledge concerning many biologi cal and immunological functions of lactoferrins and the relationships between their struc ture and function, we wanted to share our pleasure with the readers interested in this field. The present book. which represents a review of some of the most exciting contributions, is intended to reflect the status of our knowledge and transmit our hopes for the future devel opment of in vivo applications of natural and recombinant lactoferrins. We would like to express our gratitude to the sponsors who contributed to the or ganization of the meeting in such a pleasant place and allowed the participation of several young researchers. We would also like to thank all the participants who have answered with enthusiasm our invitation and to every one of the Laboratoire de Chimie Biologique for the constant and efficient help.

Zusammenfassung
Proceedings of the Third International Congress held in Le Touquet, France, May 5-9, 1997

Inhalt

1. Three-Dimensional Structure of Lactoferrin: Implications for Function, Including Comparisons with Transferrin.- 2. Structures of Buffalo and Mare Lactoferrins: Similarities, Differences, and Flexibility.- 3. Direct Detection and Quantitative Determination of Bovine Lactoferricin and Lactoferrin Fragments in Human Gastric Contents by Affinity Mass Spectrometry.- 4. Analysis of Bovine Lactoferrin in Whey Using Capillary Electrophoresis (CE) and Micellar Electrokinetic Chromatography (MEKC).- 5. Structural and Immunochemical Studies on Bovine Lactoferrin Fragments.- 6. Role of the First N-Terminal Basic Cluster of Human Lactoferrin (R2R3R4R) in the Interactions with the Jurkat Human Lymphoblastic T-Cells.- 7. Glycation Ligand Binding Motif in Lactoferrin: Implications in Diabetic Infection.- 8. Mouse Lactoferrin Gene: Promoter-Specific Regulation by EGF and cDNA Cloning of the EGF-Response-Element Binding Protein.- 9. Cloning of Human Genomic Lactoferrin Sequence and Expression in the Mammary Glands of Transgenic Animals.- 10. Expression of Human Lactoferrin in Transfected Rat Mammary Epithelial Cells.- 11. Restricted Spatiotemporal Expression of Lactoferrin during Murine Embryogenesis.- 12. Construction of Recombinant Chimeric Human Lactoferrin/Bovine Transferrins.- 13. The LDL-Receptor Family: Lactoferrin and Lipid Metabolism.- 14. Identification and Analysis of a Ca2+-Dependent Lactoferrin Receptor in Rat Liver: Lactoferrin Binds to the Asialoglycoprotein Receptor in a Galactose-Independent Manner.- 15. Bacterial Lactoferrin Receptors.- 16. Evidence for the Existence of a Surface Receptor(s) for Ferriclactoferrin and Ferrictransferrin Associated with the Plasma Membrane of the Protozoan Parasite Leishmania donovani.- 17. Lactoferrin Secretion into Mouse Milk: Development of Secretory Activity, the Localization of Lactoferrin in the Secretory Pathway, and Interactions of Lactoferrin with Milk Iron.- 18. Lactoferrin in Duodenal Aspirates during Childhood.- 19. Iron in Synovial Fluid: Removal by Lactoferrin and Relationship to Iron Regulatory Protein (IRP) Activity.- 20. The Gut: A Key Metabolic Organ Protected by Lactoferrin during Experimental Systemic Inflammation in Mice.- 21. The Antibacterial Activity of Lactoferrin and Neonatal E. coli Infections: A Selective and Critical Review.- 22. Host Defensive Effects of Orally Administered Bovine Lactoferrin.- 23. Antiviral Activity of Lactoferrin.- 24. Lactoferrin: Antiviral Activity of Lactoferrin.- 25. A Helical Region on Human Lactoferrin: Its Role in Antibacterial Pathogenesis.- 26. Interaction of Lactoferrin with Micrococcusspp. and Its Role in Antimicrobial Activity.- 27. Enhanced Anti-Candida Activity of Neutrophils and Azole Antifungal Agents in the Presence of Lactoferrin-Related Compounds.- 28. The Mechanism of in Vivo Bacteriostasis of Bovine Lactoferrin.- 29. The Main Systemic, Highly Effective, and Quickly Acting Antimicrobial Mechanisms Generated by Lactoferrin in Mammals inVivo: Activity in Health and Disease.- 30. Regulation by Lactoferrin of Epidermal Langerhans Cell Migration.- 31. Lactoferrin Stimulates the Mitogen-Activated Protein Kinase in the Human Lymphoblastic T Jurkat Cell Line.- 32. Effects of Orally Administered Bovine Lactoferrin on the Immune System of Healthy Volunteers.- 33. Lactoferrin and Interleukin-6 Interaction in Amniotic Infection.- 34. Inhibition of Azoxymethane Initiated Colon Tumor and Aberrant Crypt Foci Development by Bovine Lactoferrin Administration in F344 Rats.- 35. Bovine Lactoferrin and LactoferricinTM Inhibit Tumor Metastasis in Mice.-36. Lactoferrin Is Synthesized by Mouse Brain Tissue and Its Expression Is Enhanced after MPTP Treatment.- 37. A Study of Lactoferrin and Antibodies against Lactoferrin in Neurological Diseases.- 38. Antibodies to Lactoferrin: A Possible Link between Cow's Milk Intolerance and Autoimmune Disease.- 39. Prevalence and Clinical Significance of Anti-Lactoferrin Autoantibodies in Inflammatory Bowel Diseases and Primary Sclerosing Cholangitis.- 40. Effect of Lactoferrin on the Phagocytic Activity of Polymorphonuclear Leucocytes Isolated from Blood of Patients with Autoimmune Diseases and Staphylococcus Aureus Allergy.- 41. Lactoferrin: Its Role in Maturation and Function of Cells of the Immune System and Protection against Shock in Mice.- 42. Immunohistochemical Demonstration of Lactoferrin in Human Neoplastic Tissues.- 43. Summary.