Adipose-Derived Stem Cells

During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology™ series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.

Focuses on human adipose-derived stem cells with additional coverage of ASCs of small and large animal species Features detailed protocols accessible to new students and indispensible to experienced researchers Includes tips from the expert contributors on troubleshooting and implementation advice Includes supplementary material: sn.pub/extras

Inhalt
Isolation and Culture of Rhesus Adipose-Derived Stem Cells.- Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates.- Isolation of Murine Adipose-Derived Stem Cells.- In vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and Differentiation.- In vitro Adult Canine Adipose Tissue-Derived Stromal Cell Growth Characteristics.- Adipogenic Differentiation of Adult Equine Mesenchymal Stromal Cells.- Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells.- Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells.- Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells.- Characterization of Human ASCs Using Flow Cytometry.- Evaluation of Cellular and Humoral Immune Responses to Allogeneic Adipose-Derived Stem/Stromal Cells.- Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants.- Gel-Based and Gel-Free Proteomic Technologies.- Adipogenic Differentiation of Adipose-Derived Stem Cells.- 3D Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells.- Neural Differentiation of Human ASCs.- Osteogenic Differentiation Strategies for Adipose-Derived Mesenchymal Stem Cells.- Generation of Adipose Stromal Cell-Derived Hepatic Cells.- Adipose Stem Cell Differentiation into Smooth Muscle Cells.- Endothelial and Cardiac Regeneration from Adipose Tissues.- Epithelial Differentiation of Human Adipose-Derived Stem Cells.- Screening for Epigenetic Target Genes that Enhance Reprogramming Using Lentiviral Delivered shRNA.- Adipogenic Differentiation of Human Adipose-Derived Stem Cells on 3D Silk Scaffolds.- A First Approach for the Production of Human Adipose Tissue-Derived Stromal Cells for Therapeutic Use.- Viral Transduction of Adipose-Derived Stem Cells.- Use of Adipose-Derived Stem Cells in High Throughput Screening to Identify Modulators of Lipogenesis.- Preservation Protocols For Human Adipose Tissue-Derived Adult Stem Cells.- Soft Tissue Reconstruction.- Methods for Analyzing MicroRNA Expression and Function During Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells.- The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells.- Cell Sheet Technology for Tissue Engineering: The Self-Assembly Approach Using Adipose-Derived Stromal Cells.- Differentiation of Adipose-Derived Stem Cells for Tendon Repair.- Adipose-Derived Stem Cells for Skin Regeneration.- Adipose-Derived Stem Cells for Periodontal Tissue Regeneration.