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Adhesion Protein Protocols

  • Kartonierter Einband
  • 244 Seiten
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The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge techniques that can be adapted ... Weiterlesen
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The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge techniques that can be adapted easily to different molecules and cell types. The protocols discussed in this volume are suitable for both novice and expert scientists.

Novel techniques for studying cell-cell adhesion

The latest methods for laboratory use of microarray techniques

Adhesion protein proteomics

Includes supplementary material: sn.pub/extras

Methods in Molecular Biology Volume 96: Adhesion Protocols Editor: Dr Amanda S. Coutts Table of Contents Preface 1. Manipulation of cell-cell adhesion using bowtie-shaped microwells. Celeste M. Nelson, Wendy F. Liu, and Christopher S. Chen. 2. Separation of cell-cell adhesion complexes by differential centrifugation. Roger Vogelmann and W. James Nelson. 3. Analysis of neutrophil chemotaxis. Paul A. Nuzzi, Mary A. Lokuta, and Anna Huttenlocher. 4. Analysis of leukocyte migration through monolayers of cultured endothelial cells. Helen M. McGettrick, Lynn M. Butler, and Gerard B. Nash. 5. Biochemical purification of pseudopodia from migratory cells. Yingchun Wang and Richard L. Klemke. 6. Dynamic assessment of cell-matrix mechanical interactions in 3-D culture. W. Matthew Petroll. 7. Quantitative Analyses of Cell Adhesion Strength. Nathan D. Gallant and Andrés J. García 8. Using RNA interference to knockdown the adhesion protein TES. Elen Griffith. 9. Assaying calpain activity. Neil O. Carragher. 10. Analysis of focal adhesions and cytoskeleton by custom microarray. Matthew J. Dalby and Stephen J. Yarwood. 11. Proteomic analysis of cell surface membrane proteins in leukemic cells. Robert S. Boyd, Martin J.S. Dyer, and Kelvin Cain. 12. Bioinformatic analysis of adhesion proteins. Josephine C. Adams and Juergen Engel. 13. Analysis of integrin dynamics by Fluorescence Recovery after Photobleaching. Bernard Wehrle-Haller. 14. Double-Hydrogel Substrate as a Model System for 3D Cell Culture. Karen A. Beningo and Yu-li Wang. 15. In vitro actin assembly assays and purification from Acanthamoeba. J. Bradley Zuchero. Subject Index


Titel: Adhesion Protein Protocols
EAN: 9781617376252
ISBN: 1617376256
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 244
Gewicht: 360g
Größe: H229mm x B152mm x T13mm
Jahr: 2010
Untertitel: Englisch
Auflage: Softcover reprint of hardcover 2nd ed. 2007

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