Willkommen, schön sind Sie da!
Logo Ex Libris

Adhesion Protein Protocols

  • Fester Einband
  • 248 Seiten
(0) Erste Bewertung abgeben
Alle Bewertungen ansehen
The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can b... Weiterlesen
CHF 192.00
Print on Demand - Auslieferung erfolgt in der Regel innert 4 bis 6 Wochen.
Bestellung & Lieferung in eine Filiale möglich


The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.

Novel techniques for studying cell-cell adhesion

The latest methods for laboratory use of microarray techniques

Adhesion protein proteomics

Methods in Molecular Biology Volume 96: Adhesion Protocols Editor: Dr Amanda S. Coutts Table of Contents Preface 1. Manipulation of cell-cell adhesion using bowtie-shaped microwells. Celeste M. Nelson, Wendy F. Liu, and Christopher S. Chen. 2. Separation of cell-cell adhesion complexes by differential centrifugation. Roger Vogelmann and W. James Nelson. 3. Analysis of neutrophil chemotaxis. Paul A. Nuzzi, Mary A. Lokuta, and Anna Huttenlocher. 4. Analysis of leukocyte migration through monolayers of cultured endothelial cells. Helen M. McGettrick, Lynn M. Butler, and Gerard B. Nash. 5. Biochemical purification of pseudopodia from migratory cells. Yingchun Wang and Richard L. Klemke. 6. Dynamic assessment of cell-matrix mechanical interactions in 3-D culture. W. Matthew Petroll. 7. Quantitative Analyses of Cell Adhesion Strength. Nathan D. Gallant and Andrés J. García 8. Using RNA interference to knockdown the adhesion protein TES. Elen Griffith. 9. Assaying calpain activity. Neil O. Carragher. 10. Analysis of focal adhesions and cytoskeleton by custom microarray. Matthew J. Dalby and Stephen J. Yarwood. 11. Proteomic analysis of cell surface membrane proteins in leukemic cells. Robert S. Boyd, Martin J.S. Dyer, and Kelvin Cain. 12. Bioinformatic analysis of adhesion proteins. Josephine C. Adams and Juergen Engel. 13. Analysis of integrin dynamics by Fluorescence Recovery after Photobleaching. Bernard Wehrle-Haller. 14. Double-Hydrogel Substrate as a Model System for 3D Cell Culture. Karen A. Beningo and Yu-li Wang. 15. In vitro actin assembly assays and purification from Acanthamoeba. J. Bradley Zuchero. Subject Index


Titel: Adhesion Protein Protocols
Untertitel: No Rights This Territory
EAN: 9781588295330
ISBN: 1588295338
Format: Fester Einband
Herausgeber: Humana Press
Anzahl Seiten: 248
Gewicht: 587g
Größe: H241mm x B160mm x T20mm
Jahr: 2007
Auflage: 2nd ed. 2007

Weitere Produkte aus der Reihe "Methods in Molecular Biology"