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Activity-Based Proteomics

  • Fester Einband
  • 222 Seiten
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Beschreibung

This volume focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Activity-Based Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.




Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras



Klappentext

This volume focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Activity-Based Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.



Inhalt

Activity-Based Protein Profiling From Chemical Novelty to Biomedical Stalwart.- A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity.- Activity-Based Protein Profiling with Natural Product-Derived Chemical Probes in Human Cell Lysates.- Inhibitor Discovery by Convolution Activity-Based Protein Profiling.- Activity-Based Protein Profiling in Bacteria.- Activity-Based Lipid Esterase Profiling of M. Bovis BCG at Different Metabolic States Using Tetrahydrolipstatin (THL) as Bait.- Comparative Activity-Based Flavin-Dependent Oxidase Profiling.- Activity-Dependent Photoaffinity Labelling of Metalloproteases.- Profiling the Activity of Deubiquitinating Enzymes using Chemically Synthesized Ubiquitin-Based Probes.- Profiling Protein Sumoylation using Biochemical and Mass Spectrometry-Based Research Tools.- Live Cell Imaging and Profiling of Cysteine Cathepsin Activity using a Quenched Activity-Based Probe.- Competitive Activity-Based Protein Profiling Of Serine Hydrolases: A Case Study on DAGL-Alpha.- A Protocol for Protein Profiling using Chemoselective Cleavable Linker Probes in Semi-Permeabilized Cells.- Cleavable Linkers in Chemical Proteomics Applications.- Two-Step Activity-Based Protein Profiling With the Proteasome System as Model of Study.

Produktinformationen

Titel: Activity-Based Proteomics
Untertitel: Methods and Protocols
Editor:
EAN: 9781493964376
ISBN: 978-1-4939-6437-6
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 222
Gewicht: 631g
Größe: H16mm x B261mm x T183mm
Jahr: 2016
Untertitel: Englisch
Auflage: 1st ed. 2017

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