This detailed volume presents a comprehensive technical overview of DNA nanotechnology with an emphasis on 3D DNA nanostructure de...
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This detailed volume presents a comprehensive technical overview of DNA nanotechnology with an emphasis on 3D DNA nanostructure design and applications. Coverage spans from basic design principles for DNA and RNA nanostructures to their cutting-edge applications in a variety of fields, with the book divided into basic DNA and RNA nanostructure design strategies as well as applications utilizing DNA nanostructures, including but not limited to nanomedicine, bioimaging, biosensing, nanoplasmonics, nanoelectronics, nanofabrication, crystallography, biophysics, and analytical chemistry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, 3D DNA Nanostructure: Methods and Protocols provides the most up-to-date tutorial style overviews and technical style protocols to benefit researchers in a wide variety of areas.
Includes cutting-edge methods and protocols for the study of 3D DNA nanostructure Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Inhalt Part I: DNA/RNA Structure Design 1. Designed 3D DNA Crystals Nadrian C. Seeman, Ruojie Sha, Jens Birktoft, Jianping Zheng, Wenyan Liu, Tong Wang, and Chengde Mao 2. Three-Dimensional DNA Nanostructures Assembled from DNA Star Motifs Cheng Tian and Chuan Zhang 3. Design of Wireframe DNA Nanostructures-DNA Gridiron Dongran Han 4. Complex DNA Brick Assembly Luvena Ong, Yonggang Ke, and Peng Yin 5. Computer-Aided Design of RNA Origami Structures Steffen L. Sparvath, Cody W. Geary, and Ebbe S. Andersen 6. Assembling RNA Nanoparticles Shou-Jun Xiao Part II: Applications of DNA Structures 7. DNA Functionalization of Nanoparticles Fang Lu and Oleg Gang 8. Purification Techniques for Three-Dimensional DNA Nanostructures Travis A. Meyer 9. DNA Nanostructure as Smart Carriers for Drug Delivery Xiangyuan Ouyang, Jie Chao, Shao Su, and Chunhai Fan 10. DNA G-Quadruplex-Based Assay of Enzyme Activity Zhuoliang Liu, Kaiyu He, Wang Li, Xin Liu, Xiahong Xu, Zhou Nie, and Shouzhuo Yao 11. Assembly of Multi-Enzyme Cascade on a DNA Origami Nanostructure Jinglin Fu and Tianran Li 12. Lipid Membrane Encapsulation of a 3D DNA NanoOctahedron Steven D. Perrault and William M. Shih 13. DNA-PAINT Super-Resolution Imaging for Nucleic Acid Nanostructures Mingjie Dai 14. Designing DNA Nanotube Liquid Crystals as a Weak-Alignment Medium for NMR Structure Determination of Membrane Proteins John Min, William M. Shih, and Gaëtan Bellot 15. Direct Nanofabrication Using DNA Nanostructure Feng Zhou and Haitao Liu 16. Confined Growth of Metal Nanoparticles Within 3D DNA Origami Molds Wei Sun and Jie Shen < 17. DNA-Directed Self-Assembly of Highly Ordered and Dense Single Walled Carbon Nanotube Arrays Hareem Maune and Si-ping Han 18. A Proximity-Based Programmable DNA Nanoscale Assembly Line Xiaoyan Zhang, Xiaoqiang Ding, Jianzhou Zou, and Hongzhou Gu 19. DNA Walkers as Transport Vehicles of Nanoparticles Along a Carbon Nanotube Track Jing Pan, Tae-Gon Cha, Haorong Chen, Feiran Li, and Jong Hyun Choi