3D Cell Culture

Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text, but also require the techniques described using a standard, convenient format.

Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format.

Features detailed methods for three dimensional cell culture, most notably in the formation of tissues for clinical purpose Written by experts at engineering the reproduction of a variety of 3D cell culture models Includes vital tips on troubleshooting and avoiding known protocol pitfalls

Inhalt
3D Cell Culture: A Review of Current Approaches and Techniques.- Scaffolds for Tissue Engineering and 3D Cell Culture.- Tracking Nanoparticles in Three Dimensional Tissue Engineered Models Using Confocal Laser Scanning Microscopy.- Using Immuno-Scanning Electron Microscopy for the Observation of Cell Adhesion Interaction at the Nano- and Microscale in S-phase Cells.- 3D Sample Preparation for Orthopedic Tissue Engineering Bioreactors.- Quantification of mRNA Using Real-Time PCR and Western Blot Analysis of MAPK Events in Chondrocyte/Agarose Constructs.- Genetic Modification of Chondrocytes Using Viral Vectors.- Stem Cell and Neuron Co-Cultures for the Study of Nerve Regeneration.- Production of Tissue Engineered Skin and Oral Mucosa for Clinical and Experimental Use.- Three-Dimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for Peripheral Nerve Repair.- Encapsulation of Human Articular Chondrocytes into 3D Hydrogel: Phenotype and Genotype Characterization.- Micro-Structured Materials and Mechanical Cues in 3D Collagen Gels.- Organotypic and 3D Reconstructed Cultures of the Human Bladder and Urinary Tract.- Ex Vivo Organ Culture of Human Hair Follicles: A Model Epithelial - Neuroectodermal - Mesenchymal Interaction System.- Human Endothelial and Osteoblast Co-Cultures on 3D Biomaterials.- Assessment of Nanomaterials Cytotoxicity and Internalization.- Practical Aspects of OCT Imaging in Tissue Engineering.- Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture.- 3D Structuring of Biocompatible and Biodegradable Polymers via Stereolithography.- Alvetex®: Polystyrene Scaffold Technology for Routine Three Dimensional Cell Culture.