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2D PAGE: Sample Preparation and Fractionation

  • Fester Einband
  • 356 Seiten
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Leseprobe
This book sets forth principles and recent developments of two-dimensional electrophoresis in expression proteomics. Tricks of the... Weiterlesen
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Beschreibung

This book sets forth principles and recent developments of two-dimensional electrophoresis in expression proteomics. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time.

Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enable you to quickly learn how to perform the methods yourself. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.



Contains step-by-step instructions that ensure successful results

Provides tricks of the trade and notes on troubleshooting and avoiding known pitfalls



Inhalt
Sample Preparation and Fractionation: Volume 2 1 Application of fluorescence dye saturation labelling for differential proteome analysis of 1000 microdissected cells from pancreatic ductal adenocarcinoma precursor lesions (Kai Stühler) 2 Albumin and immunoglobulin depletion of human plasma (Rosalind E. Jenkins) 3 Multi-component Immunoaffinity Subtraction and Reversed-phase Chromatography of Human Serum (James Martosella) 4 Immunoaffinity Fractionation of Plasma Proteins by Chicken IgY Antibodies (Xiangming Fang) 5 Proteomics of Cerebrospinal Fluid: Methods for Sample Processing (John E. Hale) 6 Sample Preparation of Bronchoalveolar Lavage Fluid (Ruddy Wattiez) 7 Preparation of nasal secretions for proteome analysis (Begona Casado) 8 Preparation of Urine Samples for Proteomic Analysis (Rembert Pieper) 9 Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis (Qing-Yu He) 10 Sample Preparation of Culture Medium from Madine-Darby Canine Kidney Cells (Daniel Ambort) 11 Sample Preparation for Mass Spectrometry Analysis of Formalin-Fixed Paraffin- Embedded Tissue (Timothy D. Veenstra) 12 Metalloproteomics in the Molecular Study of Cell Physiology and Disease (Hermann-Josef Thierse) 13 Protein extraction from green plant tissue (Ragnar Flengsrud) 14 The Terminator, a device for high-throughput extraction of plant material (Bart van den Berg) 15 Isolation of mitochondria from plant cell culture (A. Harvey Millar) 16 Isolation and Preparation of Chloroplasts from Arabidopsis thaliana Plants (Paul Jarvis) 17 Isolation of plant cell wall proteins (Rafael Pont-Lezica) 18 Isolation and Fractionation of the Endoplasmic Reticulum from Castor Bean (Ricinus communis) Endosperm for Proteomic Analyses (William J Simon) 19 Cell Wall Fractionation for Yeast and FungalProteomics (Concha Gil) 20 Collection of Proteins Secreted from Yeast Protoplasts in Active Cell Wall Regeneration (Concha Gil) 21 Sample preparation procedure for cellular fungi (Alois Harder) 22 ISOLATION AND ENRICHMENT OF SECRETED PROTEINS FROM FILAMENTOUS FUNGI (Wilson A. Francisco) 23 Isolation and Solubilization of Cellular Membrane Proteins from Bacteria (Kheir Zuobi-Hasona) 24 Isolation and Solubilization of Gram-Positive Bacterial Cell Wall-Associated Proteins (Mark J. Walker) 25 Cell fractionation of parasitic protozoa (Wanderley de Souza)

Produktinformationen

Titel: 2D PAGE: Sample Preparation and Fractionation
Untertitel: Volume 2
Editor:
EAN: 9781603272094
ISBN: 1603272097
Format: Fester Einband
Herausgeber: Humana Press
Anzahl Seiten: 356
Gewicht: 699g
Größe: H241mm x B160mm x T24mm
Jahr: 2008
Untertitel: Englisch
Auflage: 2008

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