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HPLC and FPLC: Troubleshooting and Standardizing Chromatogram Purification Profiles

T. S. Amar Anand Rao

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Scientific Study from the year 2012 in the subject Biology - Micro- and Molecular Biology, Indian Institute of Science, course: M...

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Scientific Study from the year 2012 in the subject Biology - Micro- and Molecular Biology, Indian Institute of Science, course: Molecular Biology, language: English, abstract: This is a tool to separate compounds according to its hydrophobicity or hydrophilicity. A gradient is given from hydrophilic to hydrophobic range of solvent moving through a solid matrix. Here are some valuable tips collected through experience and handed over so that the reader can find it useful when the chromatograms and the instruments prove so cross with you. The instrumentation and working for both the systems HPLC and FPLC are almost same. HPLC is High Performance/Pressure Liquid Chromatography and FPLC is Fast Protein Liquid Chromtography.