Zebrafish: A Model for Marine Peptide Based Drug Screening

This book offers a comprehensive overview of toxicology, highlighting the significance of peptide-based toxins from marine environments. It discusses the principles of protein-carbohydrate and domain-domain interactions to increase our understanding of toxicology in zebrafish models, as well as drug interaction mechanisms and target definition in drug discovery. It also reviews the structure of marine peptides/toxins and the toxicology of peptide secreting cells and cells that respond to these enzymes, and describes the normal and abnormal toxicology of marine peptides in zebrafish models. Offering insights into the field of proteomics, particularly current practice and research models for solving its many riddles, the book also explains the analytical principles of marine protein-protein and protein-carbohydrate interaction in the context of teratogenicity in target identification in peptide- based drug discovery. Lastly, the book methodically examines the preclinical research on marine proteins/peptides.

Autorentext
Dr. Saravanan Ramachandran is an Assistant Professor of Marine Pharmacology at the Faculty of Allied Health Sciences, Chettinad University Chennai, India. He completed his PhD in Marine Biotechnology at Annamalai University, India. His research interests focus on drugs from marine organisms. He has received two research awards from the Indian Council of Medical Research and the Centre for Marine Living Resources and Ecology, Kochi, India, and he has published 40 research articles in international/national journals. He is a member of Society for Biotechnologists (India), International Neurotoxin Association, holds 2 patents and 1 copyright and has submitted 15 gene sequences to the NCBI. Currently, he is serving as the principal investigator of a research project funded by the Govterment of India Department of Biotechnology.

Dr. Senthilkumar Rajagopal is an Assistant Professor at the Department of Biochemistry, School of Life Sciences at Rayalaseema University, Kurnool, India. He completed his PhD in Biochemistry at Annamalai University, India, and his postdoctoral fellowship at various Universities in the USA, including the University of Virginia, Harvard and Virginia Commonwealth University. Dr. Rajagopal is a recipient of the Rameshwardasji Birla Smarak Kosh Endowment award from Mumbai Medical Trust, and the Ramalingaswami Re-entry Fellowship from the Department of Biotechnology, Ministry of Science and Technology, Government of India. His research has addressed protein kinase C modulation of calcium channels currents, and was the principal investigator of a major project entitled G-protein coupled receptor mediated intracellular mechanisms by alcohol-induced digestive disorders in the gastrointestinal tract. Other interests include G-protein coupled receptor signaling pathways in smooth muscle physiology and neurotransmitters transporters to central nervous system. Dr. Rajagopal is a life member of a number of respected scientific associations, such as the Indian Society for Atherosclerosis Research (ISAR), The Indian Science Congress Association (ISCA), Society for Free Radical Research India (SFRR), The Indian Society of Cell Biology (ISCB), the American Association of Pharmaceutical Scientists (AAPS) and the Science Advisory Board (SAB), USA.

Inhalt
Chapter 1. Biomedical importance of marine peptides/toxins.- Chapter 1.1. General Introduction peptides/toxins.- Chapter 1.2. Source/extraction of marine peptides.- Chapter 1.3. General Classification.- Chapter 1.4. Structural characterization and sequencing.- Chapter 1.5. Mode of action of marine peptides.- Chapter 1.6. Other beneficial bioactivities of marine peptides.- Chapter 2. Toxicity of peptides in Zebrafish embryos.- Chapter 2.1. Introduction to Zebrafish embryos.- Chapter 2.2. Developmental stages of Zebrafish embryos.- Chapter 2.3. Zebrafish embryo toxicity.- Chapter 2.4. Effect of peptides in Zebrafish embryos.- Chapter 2.5. Summary.- Chapter 3. Teratogenic activity of toxins in Zebrafish model.- Chapter 3.1. Introduction to toxins.- Chapter 3.2. Zebrafish embryo metamorphosis.- Chapter 3.3. Zebrafish embryo toxicity.- Chapter 3.4. Effect of toxins in Zebrafish embryos.- Chapter 3.5. Summary.- Chapter 4. Anticancer properties marine peptides/toxins using Zebrafish model.- Chapter 4.1. Introduction.- Chapter 4.2. In vivo anticancer activity of peptides.- Chapter 4.3. Gene expression and regulation.- Chapter 4.4. Effect of peptides/toxins in pathophysiology (Renal, Liver, Heart, Brain, etc).- Chapter 4.5. Summary.- Chapter 5. Protective effect of marine peptides/toxins in CVD using Zebrafish model.- Chapter 5.1. Introduction.- Chapter 5.2. Induction of CVD in Zebrafish model.- Chapter 5.3. Pharmacological agents targeting marine peptides and its clinical benefits.- Chapter 5.4. Pharmacological agents targeting peptides in receptors of myocardium and its clinical benefits.- Chapter 5.5. Conclusive summary and future challenges and opportunities