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Pancreatic Islet Isolation

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Pancreatic islets make up the endocrine pancreas and they contain the only source of insulin in the body, beta cells. Hence, acce... Weiterlesen
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Pancreatic islets make up the endocrine pancreas and they contain the only source of insulin in the body, beta cells. Hence, access to high quality preparations of pancreatic islets is fundamental for in vitro studies and to test pre-clinical applications in animal models in vivo. Access to healthy human islets is also crucial to improve transplantation procedures for diabetes. Given the susceptibility of pancreatic islets to the enzymatic digestion and mechanical stress required to obtain them, the isolation of islets is often considered as the delicate "work of a craftsman". This book, which is aimed at beginners and experts alike, is a survey of the current state-of-the-art in this field and it centres on the challenges, pitfalls and peculiarities of pancreatic islet isolation in the different species used in pre-clinical and clinical applications. It explores the similarities and differences between human islets and those from other relevant species (rodents, pigs and non-human primates), and how these influence islet isolation. The ultimate goal of this book is to improve the outcome of islet isolation and transplantation in pre-clinical and clinical applications.

Miriam Ramírez-Domínguez, University of the Basque Country, Faculty of Medicine, Laboratory of Cell Therapy of Diabetes, Leioa, Spain


Historical Background of Pancreatic Islet Isolation
Miriam Ramírez-Domínguez

The Different Faces of the Pancreatic Islets
Midhat H.Abdulreda, Rayner Rodriguez-Diaz, Over Cabrera, Alejandro Caicedo, Per-Olof Berggren

Isolation of Mouse Pancreatic Islets of Langerhans
Miriam Ramírez-Domínguez

Pancreatic Islets: Methods for Isolation and Purification of Juvenile and Adult Pig Islets
Heide Brandhorst, Paul R.V. Johnson, Daniel Brandhorst

Isolation of Pancreatic Islets from NonHuman Primates
Dora M. Berman

Necessities for a Clinical Islet Program
Wayne John Hawthorne

Clinical Islet Isolation
Wayne John Hawthorne, Lindy Jane Williams, Yi Vee Chew



Titel: Pancreatic Islet Isolation
Untertitel: From the Mouse to the Clinic
EAN: 9783319398242
ISBN: 978-3-319-39824-2
Format: E-Book (pdf)
Hersteller: Springer International Publishing
Herausgeber: Springer
Genre: Biologie
Veröffentlichung: 01.09.2016
Digitaler Kopierschutz: Wasserzeichen
Dateigrösse: 7.57 MB
Anzahl Seiten: 123
Jahr: 2016

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