Human Chromosome Methodology

Human Chromosome Methodology fills the need for an authoritative and up-to-date treatise which would serve as a text and reference for advances in human cytogenetics. The book includes readily comprehensible chapters that cover each phase of laboratory investigation from the preparation of materials for sex chromatin and chromosome techniques for bone marrow, blood, skin, and gonadal specimens to the subject of autoradiography and chromosome identification. Included also are guides to microscopy and photomicrography as well as an up-to-date treatment of chromosomes in disease.
It is hoped that this volume will serve as an adequate guide to laboratory techniques and their applications for research workers, students of genetics, and members of the medical profession involved in setting up a laboratory of cytogenetics.

Inhalt

Preface
Introduction
Sex Chromatin Techniques

1. Introduction
   II. Historical Sketch
   III. Morphological Characteristics of the Sex Chromatin
   IV. Sex Chromatin Techniques
   V. Origin of the Sex Chromatin
   VI. Sex Chromatin Patterns and Sex Chromosome Complexes
   References
   Human Peripheral Blood Leucocyte Cultures
   I. General
   II. The Mononuclear Leucocyte as the Mitotic Cell in Culture
   III. Collection of Peripheral Blood Leucocytes and Preparation of Cell Inoculum
   IV. Initiation of Mitosis in Blood Cultures: The Role of Phytohemagglutinin and Other Potentially Mitogenic Agents
   V. Influence of Culture Conditions on Cell Proliferation
   VI. Preparation of Metaphase Spreads for Cytogenic Studies
   VII. Life Span of PHA Initiated Cultures: Evidence for Secondary Mitoses in Culture
   VIII. Further Application of the Leucocyte Culture System
   IX. Methods for Culturing Human Peripheral Blood Leucocytes
   References
   Direct Chromosome Preparations of Bone Marrow Cells
   I. Introduction
   II. Procedures
   III. Discussion
   References
   The Skin Culture Technique
   I. Introduction
   II. Media and Materials
   III. Step by Step Procedure
   IV. General Discussion of Method
   Appendix
   References
   Direct Handling of Germ Cells
   I. Introduction
   II. Fixing, Squashing, and Staining Gonadal Material
   III. Various Methods of Pretreatment
   IV. Interpreting the Findings
   V. Summary
   References
   Autoradiography of Human Chromosomes
   I. Introduction
   II. Methodology
   III. Synopsis and Sequence of Technical Procedures for Autoradiography of Human Chromosomes
   IV. Techniques
   Appendix
   References
   Bright Field, Phase Contrast, and Fluorescence Microscopy
   I. Introduction
   II. Theory of Light Microscopes
   III. Properties of Microscopic Lens Systems: Bright Field Microscopy
   IV. Phase Contrast Microscopy
   V. Fluorescence Microscopic Technique
   References
   Applied Photography in Chromosome Studies
   I. Introduction
   II. Photomicrography
   III. Photosensitive Materials and Processing
   Appendix
   References
   Identification of Chromosomes
   I. Morphological Identification in the Absence of Special Markers
   II. Markers and Other Aids to Chromosome Identification
   III. Nomenclature
   IV. The Human Complement
   References
   Human Chromosomes in Disease
   I. Introduction
   II. Sex Chromosomal Abnormalities
   III. Autosomal Chromosome Abnormalities
   IV. Chromosomes in Malignant Diseases
   V. Diseases with a Normal Chromosome Complement
   VI. Conclusion
   References
   Author Index
   Subject Index