High-Resolution Electrophoresis and Immunofixation

High-Resolution Electrophoresis and Immunofixation: Techniques and Interpretation, Second Edition presents the important developments in the technology of serum protein electrophoresis. This book discusses the electrophoretic patterns that one encounters when using high-resolution electrophoresis and immunofixation.

Organized into nine chapters, this edition begins with an overview of the migration of charged particles in an electrical field. This text then presents the study of nasal and aural fluids looking for cerebrospinal fluid (CSF)-specific transferrin to detect CSF leakage due to a skull fracture or tumor. Other chapters consider some detailed studies of strategies employing immunoglobulin quantification and high-resolution electrophoresis to detect monoclonal gammopathies. This book discusses as well the three available strategies to enhance the efficiency of diagnosis of monoclonal gammopathies. The final chapter deals with the principles of high-resolution electrophoresis and immunofixation electrophoresis (IFE).

This book is a valuable resource for pathologists, technologists, epidemiologists, physicians, and clinicians.

Inhalt

Preface to the First Edition

Preface to the Second Edition

1. Methods and Rationale for High-Resolution Electrophoresis

   Review of Protein Structure and Electrophoretic Techniques

   References

2. Techniques for High-Resolution Electrophoresis

   Principles of High-Resolution Electrophoresis

   High-Resolution Electrophoresis on Agarose

   High-Resolution Electrophoresis on Cellulosic Media

   Densitometric Scanning of High-Resolution Electrophoresis

   High-Resolution Electrophoresis Versus Standard Electrophoresis

   Cost and High-Resolution Electrophoresis

   Capillary Electrophoresis

   Two-Dimensional Electrophoresis

   References

3. Proteins in Serum Identified by High-Resolution Electrophoresis

   Proteins Identified by High-Resolution Electrophoresis

   Alpha Region

   Inter-Alpha-1, -Alpha-2 Region

   Beta Region

   Gamma Region

   References

4. Approaches to Pattern Interpretation in Serum

   Initial Processing of the Sample

   Overview of the Electrophoretic Strip

   Interpretation of the Individual Patient's Sample

   References

5. Approaches to Pattern Interpretation in Cerebrospinal Fluid and Urine

   Methods to Study CSF

   HRE Interpretation for Urine

   References

6. Immunofixation Technique

   Principles of Immunoprecipitation

   Double Diffusion in Two Directions (Ouchterlony Technique)

   Immunoelectrophoresis

   Limitations of Immunoelectrophoresis

   Immunofixation Electrophoresis

   Limitations of Immunofixation

   References

7. Conditions Associated With Monoclonal Gammopathies

   Ontogeny of B Lymphocytes

   T-Lymphocyte Development

   Conditions Associated With Monoclonal Gammopathies

   References

8. Laboratory Diagnosis of Monoclonal Gammopathies

   Kappa/Lambda Quantification and the Diagnosis of Monoclonal Gammopathies

   Formulas and Rules as Guides for Detecting Monoclonal Gammopathies

   Clues to Detecting Monoclonal Gammopathies

   Maintaining an Active File on All Patients With Monoclonal Proteins

   Screening and Follow-Up of Bence Jones Protein

   Monoclonals That May Be Difficult to Diagnose

   Final Words

   References

9. Case Studies for Interpretation

   Introduction to the Case Studies

   General Comments About Interpretation