Current Topics in Cellular Regulation

Current Topics in Cellular Regulation, Volume 13 emphasizes the basic mechanisms involved in the regulation of diverse cellular activities.

This book discusses the enzymology of aminoacyl-tRNA protein transferases, analysis of mutant phenotypes, and protein kinase reaction with pyruvate kinase as substrate. The rapid hormonal regulation of pyruvate kinase in vivo, interconvertible enzyme cascades in metabolic regulation, monocyclic cascade systems, and metabolism of fructose in the liver are also elaborated. This publication likewise covers the influence of fructose on lipid metabolism, lactose operator-repressor interaction, and regulation of nitrogenase synthesis. Other topics include the involvement of isozymes in metabolic regulation and molecular approach to the complement system.

This volume is a good reference for biologists and researchers conducting work on the advances in the general area of cellular regulation.

Inhalt

Contents
List of Contributors
Posttranslational NH2-Terminal Aminoacylation
I. Introduction
II. Enzymology of Aminoacyl-tRNA Protein Transferases
III. Analysis of Acceptor Proteins
IV. Analysis of Mutant Phenotypes
V. Discussion
Note Added in Proof
References
The Regulation of Liver Pyruvate Kinase by Phosphorylation-Dephosphorylation
I. Introduction
II. Purification and Some Properties of Liver Pyruvate Kinase
III. Phosphorylation of Liver Pyruvate Kinase in Vitro and Its Effect on the Kinetic Properties of the Enzyme
IV. Phosphorylated Site of Pig Liver Pyruvate Kinase. Subtilisin-Catalyzed Modification
V. The Protein Kinase Reaction with Pyruvate Kinase as Substrate
VI. Phosphorylation of Peptides with Structures Related to the Phosphorylatable Site of Pyruvate Kinase
VII. Dephosphorylation of Pyruvate Kinase with Phosphoprotein Phosphatase
VIII. Rapid Hormonal Regulation of Pyruvate Kinase in Vivo
IX. Conclusions
References
Interconvertible Enzyme Cascades in Metabolic Regulation
I. Introduction
II. Monocyclic Cascade Systems
III. Bicyclic Cascade Systems
IV. Multicyclic Cascade Systems
V. Discussion
References
Metabolic Effects of Fructose in the Liver
I. Introduction
II. Metabolism of Fructose in the Liver
III. Influence of Fructose on the Metabolism of Glycogen
IV. Effect of Fructose on the Catabolism of Purine Nucleotides
V. Influence of Fructose on Lipid Metabolism
VI. Effect of Fructose on Alcohol Metabolism
VII. Concluding Remarks
References
Lactose Operator-Repressor Interaction
I. Introduction
II. The Lac Repressor
III. The Lac Operator
IV. Lactose Operator-Repressor Interaction
V. Models for the Operator-Repressor Interaction and Concluding
Remarks
References
Regulation of Nitrogen Fixation
I. Introduction
II. Regulation of Nitrogenase Activity
III. Regulation of Nitrogenase Synthesis
IV Electron Transport and ATP Production Coupled to N2 Reduction
V. Other Related Regulation
VI. Synopsis
References
The Role of Isozymes in Metabolism: A Model of Metabolic Pathways as the Basis for the Biological Role of Isozymes
I. Introduction
II. The Involvement of Isozymes in Metabolic Regulation
III. The Role of Compartmentation in Metabolic Regulation
IV. A Hypothetical Model of Metabolism: Polyisozymic Complexes
V. Polyisozymic Complexes in Glucose Utilization
VI. Suggested Experimental Tests of the Hypothesis
VII. Concluding Remarks
References
A Molecular Approach to the Complement System
I. Introduction
II. The Complement System
III. Individual Components
IV. Mechanisms of Action
V. Comparisons of Structure and Function
VI. Conclusions and Comments
References
Subject Index
Contents of Previous Volumes