Animal Cell Technology Meets Genomics

The 18th ESACT meeting was celebrated in Granada (Spain) in May 2003, and was entitled "Animal Cell Technology Meets Genomics", in order to reflect that the emerging technologies in the area of genomics, proteomics and other "-omics"-type disciplines will provide key technological assets to increase knowledge and open new horizons in animal cell technology. During the meeting a variety of top-class emerging technologies were presented together with the lastest advances in more mature industrial areas. The meeting was opened by a first session devoted to the understanding of basic cellular mechanisms, and four sessions focused on applied aspects of animal cell technology: Cell-based therapies and gene-based therapies, target discovery and biopharmaceuticals. The Granada Meeting has also seen a special focus on forefront industrial case studies. The spirit and scientific excellence of the 18th ESACT meeting is now reflected in different chapters of the book. The book presents, in form of short papers, a high number of the contributions to the meeting, and has been prepared with the aim to provide a relevant reference of the current research efforts in Animal Cell Technology.

Inhalt

Meeting Committees; ESACT Executive Committee; Sponsors; Companies Participating in the Trade Exhibition; Introduction: Animal Cell Technology Meets Genomics; Acknowledgements; List of Participants; CHAPTER 1: CELLULAR MECHANISMS; Effect of osmotic pressure on GS-NSO expression system, Wu M., et at.; Molecular approaches to influence epigenetic effectors of transient and stable transgene expression in mammalian cells, Hacker D. et al.; Genomic exploration on Chinese hamster ovary cells, de Leon Gatti M. et al.; Roles for glycosylation in receptor-ligand interactions in the immune system, Rudd, P.M. et al.; Investigations on mannose-6-phosphate receptor mediated protein uptake, Duerrschmid M., Jursik, et al.; Analysis of multiple apoptosis parameters using a microfluidic chip-based system, Valer M. et al.; Inhibiting apoptosis in cell culture using multiple inhibitors, Figueroa, B. et al.; Pausing of mammalian cells by cold exposure, limits and Opportunities, Hunt L. et al.; The functional competence of animal cells: analysis of the secretory pathway, Racher A.J. et al.; Proteome analysis of recombinant CHO cells under hyperosmotic stress, Lee M.S. et al.; Expression profiling analysis of sodium butyrate-induced Chinese hamster ovary cells in defined medium, Melville M.W. et al.; Intracellular nucleotide pools for optimizing productoriented transient transfection of HEK293 cells in suspension, Bassani Molinas M.M. et al.; Impact of yeast pyruvate carboxylase on the productivity of animal host cell lines, Bollati Fogolín M. et al.; Environmental effects of lactate on High-Five(TM) insect cell Metabolism, Drugmand J.-C. et al.; Correlation of intracellular nucleotide pools to amino acid concentrations in culture media by the application of multivariate methods, Hesse F. et al.; Engineering Trichoplusia ni (High Five(TM)) insect cells to express a cytosolic pyruvate carboxlase enzyme improves the viability of the cells in batch, fed-batch and perfusion cultures, LocasM.-C. et al.; Insect cell culture medium selection and optimisation based on monitoring and economical considerations, Lecina M. et al.; Antiapoptotic activity of synthetic and natural peptides, Franek F. et al.; Effect of antiapoptotic genes expression on cell growth and monoclonal antibody productivity in a hybridoma cell line, Juanola S. et al.; Amplified dicistronic expression units mediate apoptosis protection in CHO-DG44 cells adapted for growth in serum-free media, impact on mitochondria copy number, Meents H. et al.; Effect of reduced water on the apoptotic cell death triggered by oxidative stress in pancreatic ß HIT-T15 cell, Shirahata S. et al.; Characterization of apoptosis in a CHO cell line cultivated in batch and continuous culture: Effect of medium, specific perfusion rate and chemical inhibitors, Thrift J. et al.; Hydrogen peroxide-induced cellular senescence is regulated via two different pathways, Yoshizaki K. et al.; Identification of autocrine factors influencing proliferation in serum free cultures of Trichoplusia ni cells, Eriksson U. et al.; The effects of insulin and Long(TM)R3IGF-I on Chinese hamster ovary cells: cell survival, receptor activation and second messenger pathways. Yandell C.A. et al.; Conditioned medium factors in protein-free cultures of NS0 cells, Spens E. et al.; Production of human anti-peptide antibody for clinical use by in vitro immunization, Tamura, T. et al.; Optimization of in vitro immunization protocol to produce antigen specific human monoclonal antibody by demonstrating the role of IL-10, Xu Q.H. et al.; Extracellular histone H4 from SF9 cells is antimicrobial, Calles K. et al.; Analyzing calcium phosphate transfection of adherent CHO cells using microscopic live imaging, Grosjean F. et al.; Effects of multiplicity of infection (MOI) and cell cycle on Baculovirus infection kinetics, Haas R. et al.; Non-freezing preservation and subsequent recovery of hepatocytes under pO2- and pH-controlled conditions forcontinuous supply of cells for bioartificial liver support, Iding K. et al.; Selection of MDCK subclones with various genotypic and phenotypic markers: sensitivity to influenza virus infection, Kessler N. et al.; Extracellular metalloproteases and their inhibitors expressed by the IFN-ã producing CHO-320 cell line, Mols J. et al.; Novel small-molecule AKT1 inhibitors discovered by Redistribution(TM)-based high-throughput screening. Præstegaard M. et al.; Influence of mistletoe extracts and its components on invitro physiology of cancer cells, Sidler F. et al.; Rapid in-process monitoring of antibody integrity, purity and titre-2100 Bioanalyser (Lab on a chip) study, Nesredin A. et al.; Toxicological evaluation of haine river waste-water samples to appropriately sensitised cultured Fathead Minnow cells compared with the microtox® method, Dierickx P.J. et al.; CHAPTER 2: CELL BASED THERAPIES; 3D-cultivation and characterisation of osteogenic cells for the production of highly viable bone tissue implants, Barthold M. et al.; Carbon source has a considerable influence on extracellular matrix formation, demonstrated on specific collagen expression of primary human tenocytes, Möbest D. et al.; Design and characterization of cardiomyocyte-derived micro issues, Kelm M.J. et al.; Process development for standardized generation of monocyte-derived dendritic cells: applicability in breast cancer immunotherapy, Bohnenkamp H.R. et al.; Stemline(TM) hematopoietic stem cell expansion medium, a serum-free medium for expansion of CD34+ hematopoietic stem cells and progenitors, Leugers S. et al.; Mesenchymal stem cells: a very first step towards large scale cell culture strategies transplantation, Bensellam M. et al.; Expansion of murine embryonic stem cells on microcarriers-functional characteristics and scale-up potential, Burg M. et al.; In vitro cultivation of rabbit mesenchymal stromal cells on 3D bioresorbable calcium phosphate scaffolds for the generation of bone tissueimplants, Fargali S. et al.; Establishment and characterization of human medullary thyroid carcinoma cell lines for immunotherapy, Stradler G. et al.; Suppression of cell growth by platinum nanocolloids as scavengers against reactive oxygen species, Hamasaki T. et al.; Comparison of fluidized bed and fed batch reactor cultures for production of anti-HIV-antibody, Hinterleitner P. et al.; Suppression of oxidative stress-induced apoptosis of neuronal cells by electrolyzed-reduced water, Kashiwagi T. et al.; Spheroid formation by encapsulation of cancer cells to mimic small size tumors, Marc A. et al.; Bioreactor for continuous biomechanical characterization of cellular systems and tissue-engineered biohybrid tissues, Möbest D. et al.; Membrane-separated cocultivation of cord blood hematopoiet…