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Probing Protein Adsorption on Model Surfaces

  • Kartonierter Einband
  • 124 Seiten
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The ability to control nonspecific protein adsorption at interfaces is a key issue in the design of biosensors, assays, chromatogr... Weiterlesen
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Beschreibung

The ability to control nonspecific protein adsorption at interfaces is a key issue in the design of biosensors, assays, chromatographic separations and implant materials. The research presented in this manuscript focuses on the effect of surface composition on the properties of adsorbed protein layers and the probing of protein-protein interactions both on adsorbed surfaces and in solution. Monolayers of alkanethiols on gold formed from solution self-assembly and microfluidic patterning using poly(dimethyl) siloxane (PDMS) served as platform for protein adsorption. We are employed surface plasma resonance (SPR) imaging and infrared reflection absorption spectroscopy (IRRAS) to probe the conformation and orientation of adsorbed proteins, as well as, the characterization of protein layers.

Autorentext

Francis Nsiah After graduating in Chemistry from University of Cape Coast, Ghana, Dr. Nsiah went on to University of Alberta, Canada, where he obtained his MSc and PhD by carrying out research into surface characterization of thin films and protein-protein interactions. Mark T. McDermott is Chemistry Professor at the University of Alberta, Canada.

Produktinformationen

Titel: Probing Protein Adsorption on Model Surfaces
Untertitel: Sensitive Spectroscopic Approach
Autor:
EAN: 9783639100594
ISBN: 978-3-639-10059-4
Format: Kartonierter Einband
Herausgeber: VDM Verlag
Genre: Sonstiges
Anzahl Seiten: 124
Gewicht: 201g
Größe: H220mm x B150mm x T7mm
Veröffentlichung: 01.11.2008
Jahr: 2008